Synthesis of silver nanoparticles (AgNPs) from endophytic fungi is a less explored field and the present investigation is aimed at the synthesis of bioactive silver nanoparticles using endophytic fungi, Penicillium citrinum CGJ-C1 isolated from ethno-medicinal plant Tragia involucrata Lin., The AgNPs were characterized using UV-visible spectroscopy, high resolution transmission electron microscopy (HR-TEM), X-ray diffraction spectroscopy (XRD), and Fourier transform infrared spectroscopy (FTIR) methods. Spectral studies showed that biogenic AgNPs were spherical shaped and well- dispersed nanostructures with an average particle size of 2 to 5 nm. AgNPs showed cytotoxic activity against three cancer cell lines namely MCF-7, A431, and HePG2 while was nontoxic to the noncancerous cell line (HEK-293). The cytotoxic activity of these particles was found to be dose dependent. The DNA fragmentation study showed the highest DNA damage in cancer cells (MCF-7) than the non-cancerous cell line (HEK-293). The AgNPs also showed potential larvicidal activity. Hence, this study for the first time demonstrates the possibility of exploiting mycoAgNPs in the control of cancer as well as the mosquito-borne diseases.

由内生真菌合成银纳米颗粒（AgNPs）的研究较少，本研究旨在利用内生真菌从民族药用植物茶树中分离出的柠檬青霉CGJ-C1合成生物活性银纳米颗粒。使用紫外线可见光谱，高分辨率透射电子显微镜（HR-TEM），X射线衍射光谱（XRD）和傅里叶变换红外光谱（FTIR）方法对AgNP进行表征。光谱研究表明，生物型AgNPs为球形且分散良好的纳米结构，平均粒径为2至5 nm。AgNPs对三种癌细胞系MCF-7，A431和HePG2表现出细胞毒活性，而对非癌细胞系（HEK-293）无毒。发现这些颗粒的细胞毒性活性是剂量依赖性的。DNA片段研究显示，癌细胞（MCF-7）中的DNA损伤高于非癌细胞系（HEK-293）。AgNP也显示出潜在的杀幼虫活性。因此，