Chitosan is a natural polysaccharide with unique physical, chemical and biological properties that potentiates its use in many biomedical applications. In this study, chitosan thin film was doped with different concentrations of green synthesized silver nanoparticles (AgNPs) (100-400 μg) to improve its mechanical and antimicrobial activity. Transmission electron microscopy (TEM), UV-Vis absorption spectroscopy and dynamic light scattering (DLS) were used to characterize the silver nanoparticles. The silver-doped chitosan films were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) in addition to assessment of their mechanical and antimicrobial properties. The characterization results revealed the successful formation of spherical silver nanoparticles with a size distribution of 100 nm ± 40 nm. The mechanical properties of chitosan film doped with silver nanoparticles (300, 400 μg) showed superior mechanical properties over pure chitosan film. Compared with pure chitosan film, silver nanoparticles doped chitosan films showed significant antibacterial activity against Staphylococcus aureus. Chitosan film doped with 300 μg AgNPs showed significant antifungal activity against Candida albicans compared with pure chitosan. Accordingly, doping of chitosan film with silver nanoparticles greatly improves its mechanical properties and antimicrobial activity.

壳聚糖是一种天然多糖，具有独特的物理，化学和生物学特性，可增强其在许多生物医学应用中的使用。在这项研究中，壳聚糖薄膜掺杂了不同浓度的绿色合成银纳米颗粒（AgNP）（100-400μg），以提高其机械和抗菌活性。使用透射电子显微镜（TEM），紫外-可见吸收光谱和动态光散射（DLS）表征银纳米颗粒。除评估其机械和抗菌性能外，还通过扫描电子显微镜（SEM）和傅立叶变换红外（FTIR）对掺杂银的壳聚糖膜进行了表征。表征结果表明成功形成了尺寸为100 nm±40 nm的球形银纳米颗粒。掺杂有银纳米颗粒（300，400μg）的壳聚糖膜的机械性能显示出优于纯壳聚糖膜的机械性能。与纯壳聚糖膜相比，掺杂纳米银的壳聚糖膜对金黄色葡萄球菌具有显着的抗菌活性。与纯壳聚糖相比，掺杂有300μgAgNP的壳聚糖膜对白色念珠菌具有显着的抗真菌活性。因此，用银纳米粒子掺杂壳聚糖膜大大改善了其机械性能和抗菌活性。与纯壳聚糖相比，掺杂有300μgAgNP的壳聚糖膜对白色念珠菌具有显着的抗真菌活性。因此，用银纳米粒子掺杂壳聚糖膜大大改善了其机械性能和抗菌活性。与纯壳聚糖相比，掺杂有300μgAgNP的壳聚糖膜对白色念珠菌具有显着的抗真菌活性。因此，用银纳米粒子掺杂壳聚糖膜大大改善了其机械性能和抗菌活性。