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Endopolyploidy pattern in Corydalis early spring geophytes
Flora ( IF 1.7 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.flora.2020.151651
Vladislav Kolarčik , Viera Fráková , Valéria Kocová , Lukáš Koprivý , Pavol Mártonfi

Abstract Endopolyploidy, a condition where the nuclear ploidy level of various cells and tissues of an organism increases, is present in diverse plant species. Endopolyploidy contributes to cell enlargement, increases gene expression, and accelerates growth, hence it is associated with cell differentiation, development, and physiology of plants. However, the occurrence of this phenomenon in plants and the diversity of functional roles of endopolyploidy remains poorly understood. In this study, we have used flow cytometry to perform a detailed survey of the extent and patterns of endopolyploidy in various organs of two early spring geophytes, Corydalis cava and C. solida. We identified between 2C and 32C ploidy classes. Most of the stem samples contained only endopolyploid cells (4C – 32C) in both species. The endoreduplication index varied between 0.34 (leaf lamina) and 2.45 (basal part of the stem). Both species showed a similar level of endopolyploidy, and the endopolyploidy index exceeded 1.5 in the root, tuber, stem, peduncle, petiole, and scale leaf of at least one species. The endopolyploidy index differed in leaf lamina, ovary, style, and stamen between two species. Compared to other angiosperm groups, Corydalis may be included into highly endopolyploid plants. Geophytism is generally associated with high genome size, but this is not the case in Corydalis. We hypothesised that high endopolyploidy level found in Corydalis may compensate for the low genome size and thus help to promote plant growth during the early spring.

中文翻译:

紫堇属早春地质植物的内多倍体模式

摘要 内多倍体是一种生物体各种细胞和组织的核倍性水平增加的情况,存在于多种植物物种中。内多倍体有助于细胞增大,增加基因表达并加速生长,因此它与植物的细胞分化、发育和生理有关。然而,这种现象在植物中的发生以及内多倍体功能作用的多样性仍然知之甚少。在这项研究中,我们使用流式细胞术对两种早春地质植物 Corydalis cava 和 C.solida 的各个器官中内多倍体的范围和模式进行了详细调查。我们确定了 2C 和 32C 倍性类别。大多数干样本在两个物种中都只包含内多倍体细胞 (4C – 32C)。核内复制指数在 0.34(叶片)和 2.45(茎的基部)之间变化。两个物种的内多倍体水平相似,至少一个物种的根、块茎、茎、花序梗、叶柄和鳞叶的内多倍体指数超过1.5。两个物种的叶层、子房、花柱和雄蕊的内多倍体指数不同。与其他被子植物群相比,延胡索可能包含在高度内多倍体植物中。Geophytism 通常与高基因组大小有关,但在 Corydalis 中并非如此。我们假设在延胡索中发现的高内多倍体水平可以补偿低基因组大小,从而有助于促进早春植物的生长。至少一种植物的根、块茎、茎、花序梗、叶柄和鳞叶的内多倍体指数超过1.5。两个物种的叶层、子房、花柱和雄蕊的内多倍体指数不同。与其他被子植物群相比,延胡索可能包含在高度内多倍体植物中。Geophytism 通常与高基因组大小有关,但在 Corydalis 中并非如此。我们假设在延胡索中发现的高内多倍体水平可以补偿低基因组大小,从而有助于促进早春植物的生长。至少一种植物的根、块茎、茎、花序梗、叶柄和鳞叶的内多倍体指数超过1.5。两个物种的叶层、子房、花柱和雄蕊的内多倍体指数不同。与其他被子植物群相比,延胡索可能包含在高度内多倍体植物中。Geophytism 通常与高基因组大小有关,但在 Corydalis 中并非如此。我们假设在延胡索中发现的高内多倍体水平可以补偿低基因组大小,从而有助于促进早春植物的生长。延胡索可包含在高度内多倍体植物中。Geophytism 通常与高基因组大小有关,但在 Corydalis 中并非如此。我们假设在延胡索中发现的高内多倍体水平可以补偿低基因组大小,从而有助于促进早春植物的生长。延胡索可包含在高度内多倍体植物中。Geophytism 通常与高基因组大小有关,但在 Corydalis 中并非如此。我们假设在延胡索中发现的高内多倍体水平可以补偿低基因组大小,从而有助于促进早春植物的生长。
更新日期:2020-09-01
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