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Cerium metal organic framework mediated molecular threading for point-of-care colorimetric assays.
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2020-06-29 , DOI: 10.1016/j.bios.2020.112406
Xiaowei Luan 1 , Yongchun Pan 1 , Dongtao Zhou 1 , Bangshun He 2 , Xinli Liu 1 , Yanfeng Gao 1 , Jingjing Yang 1 , Yujun Song 1
Affiliation  

This work prepared a Cerium metal organic framework (Ce-MOF), which exhibits colorimetric and fluorescent response for H2O2 simultaneously. The Ce-MOF was integrated in the origami paper SlipChip to directly analyze glucose and uric acid level in serum without sample handling or extra analytical equipment except for a smartphone. Its excellent stability in wide pH and temperature, which ranges from pH 1 to 12 and from 20 to 90 °C respectively, provides wider application prospects than conventional colorimetric strategy in the horseradish peroxidase (HRP) based detection. In colorimetric assays, the “coffee ring effect” is a fatal problem which refers to the instable and inhomogeneous color rendering, and the coating of color rendering zones by dried serum proteins also seriously hinders color reading. Herein, the anchored Ce-MOF not only solves the “coffee ring effect” by modulating the mass transport, but also constructs an ingenious bio-like barrier for selectively blocking proteins in serum. To achieve that, the externally actuated strategy and Ce-MOF mediating molecular threading-dependent transport strategy were employed. Relatively good performance of our device has been demonstrated whatever in color stability and uniformity, serum protein filtration or the direct clinical sample diagnosis.



中文翻译:

铈金属有机骨架介导的即时护理比色测定的分子穿线。

这项工作制备了铈金属有机骨架(Ce-MOF),该骨架对H 2 O 2表现出比色和荧光响应同时。Ce-MOF集成在折纸SlipChip中,可直接分析血清中的葡萄糖和尿酸水平,而无需样品处理或其他分析设备(智能手机除外)。在基于辣根过氧化物酶(HRP)的检测中,其在宽广的pH和温度范围(分别为pH 1至12和20至90°C)中的出色稳定性提供了比常规比色策略更广阔的应用前景。在比色测定中,“咖啡环效应”是一个致命的问题,涉及不稳定和不均匀的显色性,而干燥的血清蛋白覆盖的显色区域也严重阻碍了颜色读取。此处,锚定的Ce-MOF不仅通过调节大众运输来解决“咖啡环效应”,而且还可以构建一种类似生物的屏障,可以选择性地阻断血清中的蛋白质。为了实现这一目标,采用了外部驱动策略和Ce-MOF介导分子线程依赖性的转运策略。无论颜色稳定性和均匀性,血清蛋白过滤还是直接临床样品诊断,我们的设备都具有相对较好的性能。

更新日期:2020-07-10
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