Abstract Neryl acetate, a monoterpenoid derivate originated from neryl diphosphate (NPP), has found extensive applications in food, agriculture, and cosmetic fields. In this study, neryl acetate was biosynthesized in recombinant Escherichia coli for the first time. First, the alcohol acetyltransferase, ATF1, was heterologously expressed in a nerol-producing E. coli strain. The resultant engineered strain, LZ006, accumulated 0.250 ± 0.014 mg/L of neryl acetate. Further overexpression of tHMG1, which improved the metabolic flux of MVA pathway, enhanced the yield to 8.064 ± 0.724 mg/L in LZ007, representing a 32.2-fold increase compared to that of LZ006. Finally, supplementation with 2 g/L pyruvate in the culture further increased neryl acetate production to 11.712 ± 0.653 mg/L, representing a 46.8-fold increase over that of the LZ006 strain. This study provides a novel method for the biosynthesis of neryl acetate and the strategies described here could be adapted to biosynthesize many other NPP-derived bioproducts.

中文翻译：

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用于生产醋酸橙花酯的大肠杆菌代谢工程

摘要 醋酸橙花酯是一种来源于二磷酸橙花酯（NPP）的单萜衍生物，在食品、农业和化妆品领域有着广泛的应用。本研究首次在重组大肠杆菌中生物合成醋酸橙花酯。首先，酒精乙酰转移酶 ATF1 在产橙花醇的大肠杆菌菌株中异源表达。由此产生的工程菌株 LZ006 积累了 0.250 ± 0.014 毫克/升的醋酸橙花酯。tHMG1 的进一步过表达改善了 MVA 途径的代谢通量，使 LZ007 的产量提高到 8.064 ± 0.724 mg/L，与 LZ006 相比增加了 32.2 倍。最后，在培养物中补充 2 g/L 丙酮酸进一步增加了醋酸橙花酯的产量至 11.712 ± 0.653 mg/L，比 LZ006 菌株增加了 46.8 倍。