Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Sugarcane streak mosaic virus (SCSMV), Sugarcane yellow leaf virus (SCYLV), and Sugarcane bacilliform virus (SCBV) are the major viruses infecting sugarcane worldwide. This study proposed a multiplex reverse transcription–polymerase chain reaction (RT–PCR) assay for the simultaneous detection and identification of these sugarcane viruses. Results showed that five primer pairs amplified their respective target viruses, indicating the assay specificity. The sensitivity of the assay was similar to or 10–100-folds lower than that of singleplex RT–PCR for detecting SCMV or the four other viruses, respectively. When detecting the five viruses in field sugarcane samples, the detection results of multiplex RT–PCR were approximately identical to those of singleplex RT–PCR, thereby indicating the relative reliability of the former. The proposed multiplex RT–PCR can simultaneously detect SCMV, SrMV, SCSMV, SCYLV, and SCBV in sugarcane, making it a suitable, efficient, and practical detection method for sugarcane viruses.

中文翻译：

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多重RT-PCR分析法同时检测和鉴定五种甘蔗病毒

甘蔗花叶病毒（SCMV），高粱花叶病毒（SrMV），甘蔗条纹花叶病毒（SCSMV），甘蔗黄叶病毒（SCYLV）和甘蔗杆状病毒（SCBV）是全世界感染甘蔗的主要病毒。这项研究提出了一种同时检测和鉴定这些甘蔗病毒的多重逆转录聚合酶链反应（RT-PCR）分析方法。结果显示五对引物扩增了它们各自的靶病毒，表明了测定的特异性。对于检测SCMV或其他四种病毒，该检测的灵敏度分别比单重RT-PCR的灵敏度低或低10-100倍。当检测现场甘蔗样品中的五种病毒时，多重RT-PCR的检测结果与单重RT-PCR的检测结果大致相同，从而表明前者的相对可靠性。提议的多重RT–PCR可以同时检测甘蔗中的SCMV，SrMV，SCSMV，SCYLV和SCBV，使其成为合适的检测方法，