Plant sugar transporters are key physiological elements in transporting sucrose and sensing specific sucrose signals. A knockoff of the novel sugar transporter gene (ScERD6) was developed by RACE-PCR from sugarcane, which incorporated an ORF of 1521 bp encoding a protein of 506 amino acids. The isolated ScERD6 gene was measured by 4722 bp DNA and containing 17 exons and 16 introns. It was found that the ScERD6 only had one transcript, and the ScERD6 protein consisted of potentially 12 transmembrane domains, belonging to sugar transporter protein. The promoter sequence was cloned by using genome walking, which included CAAT-boxes and TATA-boxes, as well as specific acting elements such as cis-regulatory elements involved in hormone response, seed development, environmental stresses, ACES, G-box, I-box, L-box, and rbcS-CMA7 light response element, etc. The treatments with 15% PEG and 6 °C stress can induce the expression of ScERD6 in the leaf, root, and stem. But ScERD6 expression in stem was different from that in leaf and root of sugarcane. Real-time PCR analysis uncovered the presence of ScERD6 in roots, stems, leaves, inflorescence, and buds, excessively, and the highest transcript level was observed in inflorescence, and the lowest in roots, while the average was detected in the stem. This finding predicted an important role of this gene in reproductive and floral development.

中文翻译：

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甘蔗中新糖转运蛋白基因ScERD6的分子表征和启动子分析

植物糖转运蛋白是转运蔗糖和检测特定蔗糖信号的关键生理元素。通过RACE-PCR从甘蔗中开发了一种新的糖转运蛋白基因（ScERD6）的仿制品，其中掺入了编码506个氨基酸的蛋白质的1521 bp的ORF。分离的ScERD6基因通过4722 bp DNA测量，并包含17个外显子和16个内含子。发现ScERD6仅具有一个转录本，ScERD6蛋白由潜在的12个跨膜结构域组成，属于糖转运蛋白。通过使用基因组步移法克隆启动子序列，其中包括CAAT框和TATA框，以及特定的作用元件，例如参与激素反应，种子发育，环境胁迫，ACES，G-box，I的顺式调控元件。 -box，L-box和rbcS-CMA7光响应元件等。用15％PEG和6°C胁迫处理可以诱导ScERD6在叶，根和茎中表达。但是ScERD6在茎中的表达不同于在甘蔗的叶和根中的表达。实时PCR分析发现ScERD6的存在根，茎，叶，花序和芽中的过量表达，在花序中观察到最高的转录水平，而在根中最低，而在茎中检测到平均值。这一发现预示了该基因在生殖和花卉发育中的重要作用。