Mesoporous silica, namely SBA-15, has been used for enzyme immobilization to help improve thermal stability of the enzymes and, in some cases, increase the enzymatic activity. It has been shown that morphology of SBA-15 is one of the factors influencing kinetics of enzyme adsorption. We reported, here, the adsorption kinetics and peroxidase activity of cytochrome c (cytc) in spherical SBA-15 in comparison of three different rod-like (fibrous, fiber, and rope) SBA-15 samples. The experimental adsorption profile fitted much better to the pseudo-second order than the pseudo-first order model. The maximum loading capacity of cytc was 466, 423, 390, and 352 mg/g for fibrous, rope, fiber, and spherical SBA-15, respectively. The rate constant for cytc adsorption for spherical SBA-15 was 3 times slower than rope and fiber SBA-15, and 15 times slower than fibrous SBA-15. The activity of cytc after immobilization in SBA-15 samples depended on the amount of cytc loading. The cytc loading in all SBA-15 at lower than 10 µmol/g showed higher activity than in free cytc in solution. The maximum activity of immobilized cytc was found in spherical SBA-15 at cytc loading of 1.00 µmol/g (~ 7 times higher than in free cytc). Finally, the Fe(III) at the heme group of cytc/SBA-15 samples with high activity was examined to contain high-spin state. The investigation in this work could suggest useful information for the preparation of SBA-15 for enzyme immobilization to appropriate applications, such as drug delivery.

介孔二氧化硅，即SBA-15，已被用于酶的固定化，以帮助提高酶的热稳定性，并在某些情况下，提高酶的活性。已经表明，SBA-15的形态是影响酶吸附动力学的因素之一。我们在这里报告了球形SBA-15中细胞色素c（cytc）的吸附动力学和过氧化物酶活性，与三种不同的棒状（纤维，纤维和绳索）SBA-15样品进行了比较。实验性吸附曲线比拟一阶模型拟合得更好。cytc对纤维，绳索，纤维和球形SBA-15的最大负载量分别为466、423、390和352 mg / g。球形SBA-15的cytc吸附速率常数比绳索和纤维SBA-15慢3倍，比纤维状SBA-15慢15倍。固定在SBA-15样品中后cytc的活性取决于cytc上样量。低于10 µmol / g的所有SBA-15中的cytc负载量均高于溶液中游离cytc的负载量。在球形SBA-15中，当cytc负载为1.00 µmol / g时，发现最大的固定化cytc活性（比游离cytc高约7倍）。最后，研究了高活性cytc / SBA-15样品血红素族的Fe（III）包含高自旋态。在这项工作中的调查可能会建议有用的信息，以准备SBA-15的酶固定到适当的应用，如药物输送。低于10 µmol / g的所有SBA-15中的cytc负载量均高于溶液中游离cytc的负载量。在球形SBA-15中，当cytc负载为1.00 µmol / g时，发现最大的固定化cytc活性（比游离cytc高约7倍）。最后，研究了高活性cytc / SBA-15样品血红素族的Fe（III）包含高自旋态。在这项工作中的调查可能会建议有用的信息，以准备SBA-15酶固定到适当的应用，如药物输送。低于10 µmol / g的所有SBA-15中的cytc负载量均高于溶液中游离cytc的负载量。在球形SBA-15中，当cytc负载为1.00 µmol / g时，发现最大的固定化cytc活性（比游离cytc高约7倍）。最后，研究了高活性cytc / SBA-15样品血红素族的Fe（III）包含高自旋态。在这项工作中的调查可能会建议有用的信息，以准备SBA-15酶固定到适当的应用，如药物输送。