Pseudomonas protegens is a gram-negative bacterium with an excellent biological control effect. Compared to standard cells growing in NaCl-free media, the ability of the hyperosmotic cells (450 mM NaCl) to resist high temperatures and freezing was significantly improved. It is of great significance to apply the P. protegens to elaborate on the hyperosmotic adaptation mechanism. RNA-seq was used to sequence P. protegens cultured with 0 mM and 450 mM NaCl. Comparative transcriptomic analyses of the different treatments were performed using gene ontology and the Kyoto encyclopedia of genes and genome. The results revealed that hyperosmotic stress had prominent impacts on the genes involving in multiple cellular functions. The hyperosmotic environment significantly affected carbohydrate, energy, and amino acid metabolism, as well as membrane system and cell motility. Our findings indicated that P. protegens adopted a series of approaches, including the high cytoplasmic concentrations of potassium ions and the uptake or synthesis of osmoprotectants, for surviving hyperosmotic stress. Among these, trehalose and proline synthesis appeared to be an important method to withstand prolonged hyperosmotic stress in P. protegens. These data provide crucial resource that may determine specific responses to the hyperosmotic environment in P. protegens.

假单胞菌蛋白是革兰氏阴性细菌，具有优异的生物防治作用。与在无NaCl培养基中生长的标准细胞相比，高渗细胞（450 mM NaCl）抵抗高温和冷冻的能力得到了显着提高。运用P. protegens阐述高渗适应机制具有重要意义。RNA-seq用于测序蛋白用0 mM和450 mM NaCl培养。使用基因本体论以及基因和基因组的京都百科全书，对不同治疗方法进行了比较转录组学分析。结果表明，高渗胁迫对涉及多种细胞功能的基因有显着影响。高渗环境显着影响碳水化合物，能量和氨基酸代谢，以及膜系统和细胞运动。我们的研究结果表明，P。protegens采用了一系列方法，包括高细胞质浓度的钾离子和摄取或合成渗透保护剂，以抵抗高渗胁迫。其中，海藻糖和脯氨酸的合成似乎是承受长期高渗胁迫的重要方法。体育蛋白。这些数据提供了至关重要的资源，可以确定对蛋白质原球菌对高渗环境的特异性反应。