Enzymatic hydrolysis of hemicelluloses in biomass feedstocks requires multiple enzymes for sacchari-fication to fermentable monosaccharides. Considering the high cost of enzymes, the need for enzymes in the cocktail for maximum saccharification can be minimized using a statistically significant experimental design. In the present study, response surface methodology was used to optimize the saccharification of wheat arabinoxylan and pretreatment of barley straw. A hemicellulase enzyme mixture consisting of recombinant xylanase (rPcXynC), arabinanase (rPcAra), and bifunctional β-xylosidase/L-arabinofuranosidase (rPcXyl) was used. A central composite design was used to evaluate and confirm the effectiveness and interactions of these enzymes along with reaction time. A 100% sacchari-fication of 1 g of wheat arabinoxylan was achieved using 21.85 U, 6.025 U, and 182 U of rPcXynC, rPcAra, and rPcXyl, respectively, for 11.46 h. Subsequently, using pretreated barley straw as a substrate, a maximum saccharification of 42% was achieved in 12 h using an enzyme cocktail of 25 U rPcXynC, 5 U rPcAra, and 200 U rPcXyl. The total hydrolysis of pretreated barley straw was increased to 1.71% by the addition of acetyl xylan esterase as a pretreatment factor followed by optimization of enzyme cocktail. The experimental values under the optimum conditions were consistent with the predicted values and a minimum cocktail was involved for a synergistic effect of the enzymes.

生物质原料中半纤维素的酶水解需要多种酶进行糖化以形成可发酵的单糖。考虑到酶的高成本，可以使用具有统计学意义的实验设计来最大限度地减少鸡尾酒中最大糖化所需的酶。在本研究中，响应面方法被用于优化小麦阿拉伯木聚糖的糖化和大麦秸秆的预处理。使用由重组木聚糖酶（rPcXynC），阿拉伯聚糖酶（rPcAra）和双功能β-木糖苷酶/ L-阿拉伯呋喃糖苷酶（rPcXyl）组成的半纤维素酶混合物。使用中央复合设计来评估和确认这些酶的有效性和相互作用以及反应时间。使用21.85 U，6可实现1 g小麦阿拉伯木聚糖的100％糖化。rPcXynC，rPcAra和rPcXyl分别为025 U和182 U，持续了11.46小时。随后，使用预处理的大麦秸秆作为底物，使用25 U rPcXynC，5 U rPcAra和200 U rPcXyl的酶混合物，在12小时内最大糖化率为42％。通过添加乙酰木聚糖酯酶作为预处理因子，然后优化酶混合物，将预处理的大麦秸秆的总水解度提高至1.71％。在最佳条件下的实验值与预测值一致，并且涉及最小的混合物以产生酶的协同作用。和200 U rPcXyl。通过添加乙酰木聚糖酯酶作为预处理因子，然后优化酶混合物，将预处理的大麦秸秆的总水解度提高至1.71％。在最佳条件下的实验值与预测值一致，并且涉及最小的混合物以产生酶的协同作用。和200 U rPcXyl。通过添加乙酰木聚糖酯酶作为预处理因子，然后优化酶混合物，将预处理的大麦秸秆的总水解度提高至1.71％。在最佳条件下的实验值与预测值一致，并且涉及最小的混合物以产生酶的协同作用。