The effects of different oxygen uptake rates (OUR) on the physiological metabolism of Rhodobacter sphaeroides were investigated systematically in 50 L fermenters due to the significant influence on industrial coenzyme Q10 production under oxygen supply limitation. Meanwhile, the seriously decreased oxygen transfer rate caused by the increased broth viscosity was successfully prevented with OUR-directed continuous ammonium sulfate feeding in the late fermentation phase. The statistical analysis results showed that controlling OUR constantly at 45 ± 2.2 mmol/L/h by the oxygen supply level adjustment and the continuous ammonium sulfate feeding could greatly enhance Q₁₀ production. This OUR-Stat controlling strategy successfully achieved the maximal coenzyme Q₁₀ production (2584 ± 82 mg/L), which was 15.4% higher than that of the control. The highest specific CoQ₁₀ content was 25.9 mg/(g DCW)), and the yield of CoQ₁₀ to glucose consumption was up to 19.37 mg/g. These results demonstrated that the optimal OUR-Stat controlling strategy would be effective and economical for improving the industrial CoQ₁₀ production.

由于氧气供应限制对工业辅酶Q10的生产产生重大影响，因此在50 L发酵罐中系统地研究了不同摄氧速率（OUR）对球形球形红细菌生理代谢的影响。同时，在发酵后期通过OUR定向连续硫酸铵进料，成功地防止了由于肉汤粘度增加而导致的氧气转移速率严重降低。统计分析结果表明，通过调节供氧量和连续添加硫酸铵，将OUR恒定控制在45±2.2 mmol / L / h，可以大大提高Q _10的产生。这种OUR-Stat控制策略成功实现了最大的辅酶Q ₁₀产量（2584±82 mg / L），比对照组高15.4％。最高的特定CoQ ₁₀含量为25.9 mg /（g DCW），CoQ ₁₀的葡萄糖消耗量高达19.37 mg / g。这些结果表明，最佳的OUR-Stat控制策略对于提高工业CoQ _10的产量将是有效且经济的。