ABSTRACT

Programmed cell death 6 (PDCD6) is a well-known apoptosis regulator that is involved in the immunity of mammals. However, the effects of miRNA-mediated regulation of PDCD6 expression on apoptosis and virus infection in organisms, especially in marine invertebrates, have not been extensively explored. In this study, PDCD6 of mud crab (Scylla paramamosain) (Sp-PDCD6) was characterized. The results showed that Sp-PDCD6 contains five EF-hands domains and could suppress virus infection via apoptosis promotion. It also presented that Sp-PDCD6 was directly targeted by miR-9875 in vitro and in vivo, miR-9875 served as a positive regulator during the virus invasion. The findings indicated that the miR-9875-PDCD6 pathway possessed fundamental effects on the immune response to virus infection in mud crab. Therefore, our research provided a novel insight into the roles of both miR-9875 and PDCD6 in the regulation of apoptosis and virus defense in mud crab.

摘要

程序性细胞死亡 6 (PDCD6) 是一种众所周知的细胞凋亡调节因子，参与哺乳动物的免疫。然而，miRNA介导的PDCD6表达调节对生物体（尤其是海洋无脊椎动物）细胞凋亡和病毒感染的影响尚未得到广泛探索。在本研究中，对泥蟹（Scylla paramamosain）的 PDCD6 ( Sp -PDCD6) 进行了表征。结果表明，Sp -PDCD6含有5个EF-hands结构域，可以通过促进细胞凋亡来抑制病毒感染。研究还表明，在体外和体内， Sp -PDCD6是miR-9875直接靶向的，miR-9875在病毒入侵过程中充当正向调节因子。研究结果表明，miR-9875-PDCD6 通路对青蟹病毒感染的免疫反应具有根本性影响。因此，我们的研究为 miR-9875 和 PDCD6 在泥蟹细胞凋亡和病毒防御调节中的作用提供了新的见解。