当前位置: X-MOL 学术Biosci. Biotechol. Biochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Aggregability of β(1→4)-linked glucosaminoglucan originating from a sulfur-oxidizing bacterium Thiothrix nivea.
Bioscience, Biotechnology, and Biochemistry ( IF 1.4 ) Pub Date : 2020-06-28 , DOI: 10.1080/09168451.2020.1785838
Minoru Takeda 1 , Keiko Kondo 2 , Ryo Tominaga 1 , Honomi Mori 1 , Mana Kato 1 , Ryoji Usami 1 , Tomoi Murakami 1 , Kazuyoshi Ueda 1 , Ichiro Suzuki 1 , Masato Katahira 2, 3
Affiliation  

β-1,4-glucosaminoglucan (GG) was prepared from the sheath of a sulfur-oxidizing bacterium Thiothrix nivea. Recently, GG was found to be adsorbed by cellulose (paper) and is therefore potentially applicable as an aminating agent for cellulose. We attempted to increase the yield of GG using a fed-batch cultivation method. Furthermore, the behavior of GG molecules in water was theoretically and experimentally investigated. NMR analysis in combination with molecular dynamics calculation suggested that GG molecules tend to form soluble aggregates in water. It was experimentally revealed that the self-aggregation is enhanced by the addition of NaCl and reduced temperature. Adsorption of GG onto cellulose via hydrogen bonding was confirmed by molecular dynamics simulation. Adsorption was also promoted in the presence of NaCl but was inhibited by a reduction in temperature. Only 11% of the amino groups in the GG-treated paper was reactive, suggesting that GG molecules adsorbed by the paper were forming aggregates.



中文翻译:

β(1→4)-连接的葡糖胺葡聚糖的聚集能力来自硫氧化细菌thiothrix nivea。

从硫氧化细菌Thiothrix nivea的鞘中制备β-1,4-葡糖胺葡聚糖(GG)。最近,发现GG被纤维素(纸)吸附,因此潜在地可用作纤维素的胺化剂。我们尝试使用分批补料培养方法提高GG的产量。此外,从理论上和实验上研究了GG分子在水中的行为。NMR分析结合分子动力学计算表明,GG分子倾向于在水中形成可溶性聚集体。实验表明,通过添加氯化钠和降低温度可以增强自聚集。通过分子动力学模拟证实了GG通过氢键吸附到纤维素上。在NaCl存在下也促进了吸附,但是通过降低温度抑制了吸附。GG处理过的纸中只有11%的氨基具有反应性,

更新日期:2020-06-28
down
wechat
bug