Abstract Potato peel waste (PPW) is a carbohydrate-rich waste from potato industries, which is an environmental threat worldwide. In this study, it was evaluated for biobutanol production via acetone-butanol-ethanol fermentation by Clostridium acetobutylicum. The results showed that PPW contained a considerable amount of glycoalkaloids, severe inhibitors for the bacterium. Thus, three processes, i.e., dilute acid pretreatment (Process I), the inhibitors extraction followed by dilute acid hydrolysis (Process II) and ethanol organosolv pretreatment (Process III), were employed before hydrolysis and fermentation to produce ABE. The extraction of glycoalkaloids with ethanol, dilute acid hydrolysis at 180 °C for 60 min, and enzymatic hydrolysis led to a hydrolysate with 36 g/L glucose, which was successfully fermented to 11.6 g/L ABE. In process II, the organosolv pretreatment led to the removal of the major fraction of inhibitors, in the range of 77–88% of glycoalkaloids. The enzymatic hydrolysis of PPW pretreated with 75% ethanol at 180 °C for 60 min resulted in a fermentable hydrolysate with 38 g/L glucose. The fermentation of overall hydrolysate resulted in a high ABE concentration of 24.8 g/L, indicating that PPW is an appropriate substrate for butanol production after the removal of its bacterial inhibitors.

中文翻译：

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通过单独和同时去除抑制剂和预处理从马铃薯皮废料中高效生产生物丁醇

摘要 马铃薯皮废料 (PPW) 是一种来自马铃薯工业的富含碳水化合物的废料，是世界范围内的环境威胁。在本研究中，对丙酮丁醇梭菌通过丙酮-丁醇-乙醇发酵生产生物丁醇进行了评估。结果表明，PPW 含有大量的配糖生物碱，这是该细菌的严重抑制剂。因此，在水解和发酵以产生ABE之前采用三个过程，即稀酸预处理（过程I）、抑制剂提取然后稀酸水解（过程II）和乙醇有机溶剂预处理（过程III）。乙醇提取配糖生物碱，180 °C 稀酸水解 60 分钟，酶解得到 36 g/L 葡萄糖的水解产物，成功发酵至 11.6 g/L ABE。在过程二中，有机溶剂预处理导致主要部分抑制剂的去除，范围为 77-88% 的配糖生物碱。在 180°C 下用 75% 乙醇预处理 60 分钟的 PPW 的酶促水解产生具有 38 g/L 葡萄糖的可发酵水解产物。整个水解产物的发酵产生 24.8 g/L 的高 ABE 浓度，表明 PPW 是去除细菌抑制剂后生产丁醇的合适底物。