This work demonstrates the development of a compact, modular, cost-effective separation system configured to address a specific separation problem. The principles of the separation are based on gradient capillary liquid chromatography where the system consists of precision stepper motor-driven portable syringe pumps with interchangeable glass syringes (100 µL to 1000 µL). Excellent flow-rate precision of < 1% RSD was achieved with typical flow-rates ranging from 1 µL/min to 100 µL/min, which was ideal for capillary columns. A variable external loop volume and electrically actuated miniature injection valve was used for sample introduction. Detection was based upon a commercial Z-type UV absorbance flow-cell housed within a custom-built cooling enclosure (40mm x 40mm) which also contained a UV-LED light-source and a photodiode. System and chromatographic performance was evaluated using linear gradient elution, with day to day repeatability of <1.5 % RSD (n=6) for peak area, and < 0.4% RSD (n=6) for retention time, for the separation of a 5 component mixture using a 50mm X 530 µm ID C₁₈ 3µm particle capillary column. The system can run any commercial or in-house packed columns from 50mm to 100mm length with IDs ranging from 200 to 700µm. The developed portable system was operated using custom-built windows-based chromatography software, complete with data acquisition and system control.

这项工作演示了一种紧凑，模块化，经济高效的分离系统的开发，该系统配置为解决特定的分离问题。分离原理基于梯度毛细管液相色谱法，该系统由精密步进电机驱动的便携式注射器泵和可互换的玻璃注射器（100 µL至1000 µL）组成。优良的<在1 µL / min至100 µL / min的典型流速下，RSD达到1％，这是毛细管色谱柱的理想选择。可变的外部环路容积和电动微型注射阀用于样品引入。检测基于商业化的Z型紫外线吸收流通池，该流通池安装在定制的冷却外壳（40mm x 40mm）中，该外壳还包含一个UV-LED光源和一个光电二极管。使用线性梯度洗脱评估系统和色谱性能，峰面积的日常重复性<1.5％RSD（n = 6），保留时间<0.4％RSD（n = 6），分离5成分混合物使用50mm X 530 µm ID C ₁₈3µm颗粒毛细管柱。该系统可以运行长度从50mm至100mm的任何商业或内部填充柱，ID范围从200至700μm。开发的便携式系统使用定制的基于Windows的色谱软件进行操作，并具有数据采集和系统控制功能。