The present study describes the fabrication of 3D nucleated microparticles like Cu-MOF (MPsLCu-MOF)-SWCNTs composite on glassy carbon electrode (GCE) by electrodeposition and applied as a sensor for the sensitive determination of glucose in human saliva samples. First, SWCNTs were deposited on GCE by potential cycling between 0.0 V to -1.0 V and then, Cu-MOF was electrodeposited on SWCNTs modified GCE using a ligand, 1,3,5-benzene tricarboxylic acid (BTC), triethylamine hydrochloride and copper salt. The SEM studies showed the formation of MPsLCu-MOF on SWCNTs as well as on bare GCE with 1 μm size. The impedance studies showed that the heterogeneous electron transfer rate constant of [Fe(CN)₆]^3-/4- was higher at SWCNTs-MPsLCu-MOF than bare GCE and SWCNTs and MPsLCu-MOF modified GCEs. Then, the composite electrode was utilized for non-enzymatic detection of glucose. The SWCNTs-MPsLCu-MOF modified electrode showed a well-defined sharp oxidation peak at 0.45 V with enhanced current than bare GCE and SWCNTs and MPsLCu-MOF modified GCEs. As the concentration of glucose increases from 10-160 × 10^-6 M, the oxidation current also increases without shifting its oxidation potential. The present composite modified electrode was also used to determine glucose in the linear range of 20 × 10^-9 M to 80 × 10^-6 M by amperometry with a LOD of 1.72 × 10^-9 M (S/N=3) and a superior sensitivity of 573 μA mM⁻¹ cm⁻². Finally, the composite fabricated electrode was exploited for real-time analysis of glucose in human saliva samples and satisfactory results were obtained.

本研究描述了通过电沉积在玻碳电极（GCE）上制备3D有核微粒，如Cu-MOF（MPsLCu-MOF）-SWCNTs复合材料，并用作敏感测定人唾液样品中葡萄糖的传感器。首先，通过在0.0 V至-1.0 V之间的电势循环将SWCNT沉积在GCE上，然后，使用配体，1,3,5-苯三羧酸（BTC），三乙胺盐酸盐和铜将Cu-MOF电沉积在SWCNT修饰的GCE上盐。SEM研究表明，MPsLCu-MOF在SWCNT以及尺寸为1μm的裸GCE上形成。阻抗研究表明，[Fe（CN）₆ ] ^{3- / 4-}的异质电子传递速率常数SWCNTs-MPsLCu-MOF比裸GCE和SWCNTs和MPsLCu-MOF修饰的GCE高。然后，将复合电极用于葡萄糖的非酶检测。与裸GCE和SWCNTs和MPsLCu-MOF修饰的GCE相比，SWCNTs-MPsLCu-MOF修饰的电极在0.45 V处显示出明确定义的尖锐氧化峰，并具有增强的电流。随着葡萄糖浓度从10-160×10 ^-6 M增加，氧化电流也增加而不改变其氧化电位。本复合修饰电极还用于通过安培法在20×10 ^-9 M至80×10 ^-6 M的线性范围内测定葡萄糖，LOD为1.72×10 ^-9 M（S / N = 3）， 573μAmM ^-1  cm的出色灵敏度⁻²。最后，将该复合电极用于人唾液样品中葡萄糖的实时分析，并获得满意的结果。