Juglone and thymoquinone are cytotoxic to pancreatic cancer cells. The aim of this study was to investigate, using an analysis of isobolograms, the type and degree of interactions between juglone and thymoquinone on MIA PaCa-2 pancreatic cancer cells. Cell viability was evaluated using the MTT assay. Cell death was determined by flow cytometry. The IC₅₀ value for juglone and TQ in combination was found to be 24.75 μM, which was higher than juglone or TQ alone. Juglone alone killed Mia Paca-2 cells by ferroptosis. At concentrations where 10, 20 or 50% of cells were affected, there existed a moderate antagonistic relationship between juglone and TQ as indicated by the combination index (CI) value determined by the Compusyn software. At concentrations that affected 75% and 90% of cells, there were nearly an additive effect with CI value of 1.09249 and 0.92391, respectively. Moderate synergism was only seen at concentration where 95% of cells were affected, and the corresponding concentration of juglone and TQ at that combination was 40.90 μM and 511.19 μM, respectively.

胡桃醌和百里醌对胰腺癌细胞具有细胞毒性。本研究的目的是使用等效线图分析，研究胡桃醌和百里醌对 MIA PaCa-2 胰腺癌细胞相互作用的类型和程度。使用MTT测定评估细胞活力。通过流式细胞术确定细胞死亡。IC ₅₀胡桃醌和 TQ 组合的值为 24.75 μM，高于单独的胡桃醌或 TQ。Juglone 单独通过 ferroptosis 杀死了 Mia Paca-2 细胞。Compusyn 软件确定的组合指数 (CI) 值表明，在 10、20 或 50% 的细胞受到影响的浓度下，胡桃醌和 TQ 之间存在中度拮抗关系。在影响 75% 和 90% 细胞的浓度下，几乎存在累加效应，CI 值分别为 1.09249 和 0.92391。仅在 95% 的细胞受到影响的浓度下才观察到中度协同作用，该组合中胡桃醌和 TQ 的相应浓度分别为 40.90 μM 和 511.19 μM。