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Precision medicine in Alzheimer's disease: An origami paper-based electrochemical device for cholinesterase inhibitors.
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2020-06-28 , DOI: 10.1016/j.bios.2020.112411
Veronica Caratelli 1 , Adriana Ciampaglia 1 , Jacopo Guiducci 1 , Giuseppe Sancesario 2 , Danila Moscone 1 , Fabiana Arduini 3
Affiliation  

In the overall scenario of precision medicine, we propose a novel paper-based lab-on-a-chip to deliver a cost-effective and easy to use sensing tool for customized administration of drugs in Alzheimer’s disease. Among several drugs, we designed the device for evaluating the efficacy of compounds (e.g. physostigmine, rivastigmine, donepezil) which are able to inhibit in a reversible way the cholinesterase enzyme. Because cholinesterase activity is peculiar to each patient, the administration of customized amount of the drug can improve the treatment efficacy and the quality of patient life, avoiding side effects due to the overdosage. In detail, we exploited Vivid™ Plasma Separation membrane to threat the whole blood sample, filter paper to load the reagents needed for the measurement, and office paper to print electrodes able to measure the butyrylcholinesterase activity, delivering a reagent free analytical tool. The calibration curve of butyrylcholinesterase obtained in blood sample provided linearity between 2 and 12 U/mL, with sensitivity of 0.050 ± 0.004 μA mL/U. The physostigmine, rivastigmine, and donepezil inhibition activities toward the butyrylcholinesterase enzyme were also measured in blood sample with linearity up to respectively 0.5 μM, 25 μM, 30 μM, and detection limits of 0.009 μM, 0.4 μM, 0.3 μM. These results demonstrate the capability of paper-based origami sensors as point of care devices to customize the drug administration in Alzheimer’s disease.



中文翻译:

阿尔茨海默氏病的精密医学:一种基于折纸纸的电化学装置,用于胆碱酯酶抑制剂。

在精密医学的整体情况下,我们提出了一种新型的基于纸的纸上实验室,以提供一种经济高效且易于使用的传感工具,用于个性化阿尔茨海默氏病的药物管理。在几种药物中,我们设计了一种装置,用于评估能够以可逆方式抑制胆碱酯酶的化合物(例如毒扁豆碱,卡巴拉汀,多奈哌齐)的功效。因为胆碱酯酶的活性对每个患者而言都是独特的,所以按定制量的药物给药可以改善治疗效果和患者生活质量,避免因过量而产生的副作用。详细而言,我们利用Vivid™血浆分离膜来威胁全血样本,滤纸装载测量所需的试剂,和办公室用纸打印能够测量丁酰胆碱酯酶活性的电极,从而提供了无试剂的分析工具。血液样品中丁酰胆碱酯酶的校准曲线在2至12 U / mL之间提供线性,灵敏度为0.050±0.004μAmL / U。还测定了血样中的毒扁豆碱,卡巴拉汀和多奈哌齐对丁酰胆碱酯酶的抑制活性,线性分别高达0.5μM,25μM,30μM,检出限为0.009μM,0.4μM,0.3μM。这些结果证明了纸质折纸传感器作为护理设备的功能,可以定制阿尔茨海默氏病的药物管理方式。血液样品中丁酰胆碱酯酶的校准曲线在2至12 U / mL之间提供线性,灵敏度为0.050±0.004μAmL / U。还测定了血样中的毒扁豆碱,卡巴拉汀和多奈哌齐对丁酰胆碱酯酶的抑制活性,线性分别高达0.5μM,25μM,30μM,检出限为0.009μM,0.4μM,0.3μM。这些结果证明了纸质折纸传感器作为护理设备的功能,可以定制阿尔茨海默氏病的药物管理方式。血液样品中丁酰胆碱酯酶的校准曲线在2至12 U / mL之间提供线性,灵敏度为0.050±0.004μAmL / U。还测定了血样中的毒扁豆碱,卡巴拉汀和多奈哌齐对丁酰胆碱酯酶的抑制活性,线性分别高达0.5μM,25μM,30μM,检出限为0.009μM,0.4μM,0.3μM。这些结果证明了纸质折纸传感器作为护理设备的功能,可以定制阿尔茨海默氏病的药物管理方式。0.4μM,0.3μM。这些结果证明了纸质折纸传感器作为护理设备的功能,可以定制阿尔茨海默氏病的药物管理方式。0.4μM,0.3μM。这些结果证明了纸质折纸传感器作为护理设备的功能,可以定制阿尔茨海默氏病的药物管理方式。

更新日期:2020-06-28
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