A novel AIEgen-based probe for detecting cysteine in lipid droplets,Analytica Chimica Acta

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A novel AIEgen-based probe for detecting cysteine in lipid droplets
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.aca.2020.05.074
Wei Cheng ₁ , Xuqi Xue ₁ , Fang Zhang ₁ , Baoxin Zhang ₁ , Taihan Li ₂ , Liang Peng ₃ , Dong-Hyung Cho ₄ , Hongli Chen ₁ , Jianguo Fang ₁ , Xingguo Chen ₁

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A smart fluorescent probe DPAS-Cys has been rationally designed based on a typical AIEgen DPAS and an acrylate moiety. The probe DPAS-Cys not only can be used for the detection of cysteine (Cys) selectively with large Stokes shift (200 nm) and relatively low detection limit (2.4 μM), but also shows lipid droplets (LDs) targeting property. The response mechanism for Cys was carefully verified. Importantly, due to the aggregation-induced emission characteristic, the introduction of considerable percentage of traditional organic solvent is avoidable, which makes it suitable for bioimaging in physiological systems. In addition, the confocal fluorescence imaging demonstrates that DPAS-Cys is able to detect Cys in LDs of different cell lines with universality. Our study opens a new avenue to understand the importance of LDs in biosystem, for which the gap between the essential biothiol Cys and the energy storage organelle LDs was bridged for the first time.

中文翻译：

一种基于 AIEgen 的新型探针，用于检测脂滴中的半胱氨酸

基于典型的AIEgen DPAS和丙烯酸酯部分合理设计了智能荧光探针DPAS-Cys。探针 DPAS-Cys 不仅可以选择性地检测半胱氨酸 (Cys)，具有较大的斯托克斯位移 (200 nm) 和相对较低的检测限 (2.4 μM)，而且还具有脂滴 (LDs) 靶向性。Cys 的响应机制经过仔细验证。重要的是，由于聚集诱导发射特性，可以避免引入相当比例的传统有机溶剂，这使其适用于生理系统中的生物成像。此外，共聚焦荧光成像表明DPAS-Cys能够普遍检测不同细胞系LDs中的Cys。我们的研究为了解 LD 在生物系统中的重要性开辟了一条新途径，

更新日期：2020-08-01

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