Harvested microalgae Chlorella spp. and primary sludge were co-digested in a laboratory-scale anaerobic membrane bioreactor (AnMBR) under thermophilic conditions (55 °C). The system was run for 700 days divided into four experimental phases to determine the influence of the organic loading rate on the process performance and the microbial community. The rise in organic loading rate from 0.17 to 0.5 gCOD·L⁻¹·d⁻¹ led to a 35% improvement in methane production. The system reached 69% biodegradability working at 0.5 gCOD·L⁻¹·d⁻¹ and a high solids retention time (70 d), indicating the efficient conversion of biomass into biogas through the AnMBR configuration while avoiding possible inhibition by free ammonia. The thermophilic AnMBR provided a stable microbial community dominated by hydrolytic and fermenter members such as Coprothermobacter, Fervidobacterium and Hydrothermae. Syntrophic oxidisers such as W5 were also key in propionate degradation and produced intermediates for later conversion into methane.

收获的微藻小球藻属。在高温条件下（55°C），在实验室规模的厌氧膜生物反应器（AnMBR）中共同消化初级污泥和初级污泥。该系统运行700天，分为四个实验阶段，以确定有机负载率对工艺性能和微生物群落的影响。有机负载率从0.17 gCOD·L ^-1 ·d ^-1增加到甲烷产量提高了35％。在0.5 gCOD·L ^-1 ·d ^{-1的条件下}，系统达到了69％的生物降解性。高的固体保留时间（70 d），表明通过AnMBR构型将生物质有效转化为沼气，同时避免了游离氨的可能抑制。嗜热AnMBR提供的水解和发酵成员如主导的稳定的微生物群落Coprothermobacter，Fervidobacterium和Hydrothermae。诸如W5的同质氧化剂也是丙酸酯降解的关键，并产生了中间体，供以后转化为甲烷。