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Exploring Ulva australis Areschoug for possible biotechnological applications: In vitro antioxidant and enzymatic inhibitory properties, and fatty acids contents
Algal Research ( IF 4.6 ) Pub Date : 2020-06-27 , DOI: 10.1016/j.algal.2020.101980
Riccardo Trentin , Luísa Custódio , Maria João Rodrigues , Emanuela Moschin , Katia Sciuto , José Paulo da Silva , Isabella Moro

Ulva australis Areschoug (Ulvales, Chlorophyta) is traditionally used as a foodstuff in Korea and to treat several diseases, such as hyperlipidemia and urinary ailments. It is now considered an invasive species of the Venice Lagoon (Italy). This work explored U. australis as a potential source of bioactive molecules with biotechnological applications. For that purpose, organic and water extracts were prepared from dried biomass of U. australis and evaluated for radical scavenging activity (RSA), metal chelating activities and for in vitro inhibition of enzymes related with several disorders, namely Alzheimer's disease (acetylcholinesterase, butyrylcholinesterase), hyperpigmentation (tyrosinase), obesity (lipase), Type-2 diabetes mellitus (α-glucosidase, α-amylase) and skin sagging (elastase). Extracts were also evaluated for total contents of phenolics, flavonoids condensed tannins and carbohydrates. Finally, the fatty acids methyl esters profile of the biomass was established by gas chromatography–mass spectrometry. Extracts had low to moderate radical scavenging activity on DPPH, and a higher ability to chelate Fe2+ than Cu2+, and the maximum Fe2+ chelating capacity was observed in the tincture (IC50 = 4.82 mg/mL). Samples had a higher ability to inhibit α-amylase and microbial α-glucosidase than the other tested enzymes. The biomass of U. australis presented a highly saturated fatty acids profile, enriched in palmitic acid. These data suggest U. australis as a promising source of natural products with anti- diabetic properties and as a desirable species for biodiesel production.



中文翻译:

探索可能的生物技术应用中的澳大利亚石Ul:体外抗氧化剂和酶促抑制特性以及脂肪酸含量

Ulva australis Areschoug(Ulvales,Chlorophyta)在韩国传统上被用作食品,用于治疗多种疾病,例如高脂血症和泌尿系统疾病。现在,它被认为是威尼斯泻湖(意大利)的一种入侵物种。这项工作探索ü。在生物技术应用中,澳大利亚是生物活性分子的潜在来源。为此,从U的干燥生物质中制备了有机提取物和水提取物。芦苇并评价自由基清除活性(RSA),金属螯合活动和体外抑制与多种疾病有关的酶,即阿尔茨海默氏病(乙酰胆碱酯酶,丁酰胆碱酯酶),色素沉着(酪氨酸酶),肥胖症(脂肪酶),2型糖尿病(α-葡萄糖苷酶,α-淀粉酶)和皮肤松弛(弹性蛋白酶)。还评估了提取物的酚类,类黄酮缩合单宁和碳水化合物的总含量。最后,通过气相色谱-质谱法建立了生物质的脂肪酸甲酯分布图。提取物对DPPH具有较低至中等的自由基清除活性,并且与Cu 2+相比具有更高的螯合Fe 2+的能力,并且the剂中具有最大的Fe 2+螯合能力(IC 50)。 = 4.82 mg / mL)。样品具有比其他测试酶更高的抑制α-淀粉酶和微生物α-葡萄糖苷酶的能力。U的生物量。南澳大利亚州呈现出高度饱和的脂肪酸谱,富含棕榈酸。这些数据表明ü澳大利亚是具有抗糖尿病特性的天然产物的有前途的来源,也是生物柴油生产的理想物种。

更新日期:2020-06-27
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