Microglia are the immune cells of the central nervous system involved in a variety of developmental processes, such as regulation of cell death and survival, spatial patterning, and contribute to the development of Purkinje cells (PCs) during migration. Microglia express immunoglobulin G Fc receptors (FcgRs). In this report, we describe microglial FcgR expression and its relation to abnormal PC migration in the cerebellum during development. To detect microglial FcgR, the direct anti-IgG (secondary antisera) and high concentrations of Triton X-100 were applied as a method for labeling microglial cells without the use of any specific primary antiserum. By using Acp2^−/− mice, which show an excessive PC migration into the molecular layer (ml), and 3 different types of mice with a null to alter the Reelin pathway (Reeler-, Dab1 (SCM)-, and Apoer mutant mice), we studied the location of PCs and the expression of FcgRs. Wild type littermates were used as controls in all studies. We show that the expression of microglial FcgRs was absent and PCs were ectopically located in the white matter in the cerebella of all mutant mice, except for the Acp2^−/− mice (PCs were located in the ml). These results suggest a role for FcgRs in the Reelin signaling pathway, not in regulating PC migration, but rather in the adaptation to an environment with a relatively large number of ectopically located PCs. However, the exact correlation between the ectopic location of PCs and lack of FcgRs in Reeler, SCM, and Apoer^−/− mice and the presence of FcgRs and directed PC location in the ml in Acp2^−/− mice are yet to be determined.

小胶质细胞是中枢神经系统的免疫细胞，参与各种发育过程，例如调节细胞死亡和存活，空间模式，并在迁移过程中促进浦肯野细胞（PC）的发育。小胶质细胞表达免疫球蛋白G Fc受体（FcgRs）。在此报告中，我们描述了小胶质FcgR表达及其与发育过程中小脑中异常PC迁移的关系。为了检测小胶质细胞FcgR，直接抗IgG（次级抗血清）和高浓度的Triton X-100被用作标记小胶质细胞的方法，而无需使用任何特定的主抗血清。通过使用Acp2 ^-/-显示过度PC迁移到分子层（ml）的小鼠和无效以改变Reelin途径的3种不同类型的小鼠（Reeler-，Dab1（SCM）-和Apoer突变小鼠），我们研究了位置PCs和FcgRs的表达。在所有研究中均使用野生型同窝仔作为对照。我们显示，除了Acp2 ^-/-以外，所有突变小鼠小脑的小胶质细胞FcgRs的表达均不存在，且PCs异位位于小脑的白质中小鼠（PC位于ml中）。这些结果表明FcgR在Reelin信号传导途径中的作用，而不是在调节PC迁移中，而是在适应具有大量异位定位的PC的环境中。然而，和PC的异位位置之间的精确相关性缺乏FcgRs的均整机，SCM和Apoer ^{- / -}小鼠和FcgRs的在存在和定向PC位置在毫升ACP2 ^{- / -}小鼠是尚未确定。