The most probable number-polymerase chain reaction (MPN-PCR) method was assessed for the specific detection and quantification of Ralstonia pseudosolanacearum race 4 strains in soil from Zingiberaceae fields in Japan. Based on the genome sequence analysis of two bacterial strains [MAFF 211479 (type I) and MAFF 211472 (type II)] isolated from ginger, race 4 type I- and type II-specific nested PCR primer sets were designed, respectively. The MPN-PCR using these primer sets efficiently detected and quantified the pathogen in naturally and artificially infested soils.

中文翻译：

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MPN-PCR技术特异性检测和定量分析姜科植物栽培土壤中拟南芥Ralstonia pseudosolanacearum race 4菌株

评估了最可能的数字聚合酶链反应（MPN-PCR）方法对日本姜科土壤中的Ralstonia pseudosolanacearum第4种族菌株进行特异性检测和定量。基于从姜中分离出的两种细菌菌株[MAFF 211479（I型）和MAFF 211472（II型）]的基因组序列分析，分别设计了第4种I型和II型特异性巢式PCR引物。使用这些引物对的MPN-PCR可有效检测和定量天然和人工感染土壤中的病原体。