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Mapping of quantitative trait loci associated with resistance to brown planthopper in background of Swarna from a traditional variety PTB33
Euphytica ( IF 1.6 ) Pub Date : 2020-06-28 , DOI: 10.1007/s10681-020-02652-4
Sai Harini Akula , M. Ayyappa Dass , Sai Kumar Surapaneni , Padma Balaravi

Among all the insect pests, brown planthopper (BPH), Nilaparvata lugens (Stal) (Homoptera: Delphacidae) is one of the most destructive pests of rice causing severe yield losses. In this study, an attempt was made to map QTL associated with resistance to BPH using a RIL (F 7 ) population derived from the cross Swarna/PTB33 using standard seedling seedbox screening technique (SSST) at seedling stage, modified mass tiller screening (MMTS) at tillering stage and field screening (FS) at vegetative and reproductive stages in three replications. The mean damage score values of mapping population evaluated for BPH resistance in SSST (score 4.4), MMTS (score 4.1) and FS (score 4.6) methods were showed significantly higher resistance than the recipient parent Swarna (Score 9). Out of 327 SSR markers, 105 markers revealed polymorphism between the parents. QTL analysis detected 4 major QTL on chromosomes 1, 6 and 12 and 2 minor QTL on chromosome 3 and 6 derived from PTB33. Among 4 QTL detected QBph6.1 (RM7158-RM19606), QBph6.3 (RM402-RM276) and QBph12.1 (RM28378-RM28427) were associated with high phenotypic variance and tightly linked markers which enable their practical utility in marker assisted selection. The results indicate phenotypic analysis at sensitive stage (maximum tillering to soft dough stage) of crop growth for BPH resistance would help in improving the efficiency of QTL detection and efficient introgression of these QTL’s into elite rice cultivars, an important approach to develop stable resistance to BPH and in understanding the quantitative resistance to BPH in rice.

中文翻译:

传统品种 PTB33 的 Swarna 背景中与褐飞虱抗性相关的数量性状位点定位

在所有害虫中,褐飞虱(BPH)、褐飞虱(Stal)(同翅目:飞虱科)是对水稻危害最大的害虫之一,造成严重的产量损失。在本研究中,尝试使用源自杂交 Swarna/PTB33 的 RIL (F 7 ) 群体在幼苗阶段使用标准幼苗种箱筛选技术 (SSST)、改良分蘖筛选 (MMTS) 绘制与 BPH 抗性相关的 QTL ) 在分蘖阶段和营养和繁殖阶段的田间筛选 (FS) 三个重复。在 SSST(4.4 分)、MMTS(4.1 分)和 FS(4.6 分)方法中对 BPH 抗性评估的映射群体的平均损伤评分值显示出显着高于受体亲本 Swarna(9 分)的抗性。在 327 个 SSR 标记中,105 个标记揭示了亲本之间的多态性。QTL分析在1、6和12号染色体上检测到4个主要QTL,在3、6号染色体上检测到2个来自PTB33的次要QTL。在检测到的 4 个 QTL 中,QBph6.1 (RM7158-RM19606)、QBph6.3 (RM402-RM276) 和 QBph12.1 (RM28378-RM28427) 与高表型变异和紧密连锁的标记相关,这使得它们在标记辅助选择中具有实际效用。结果表明,在作物生长敏感阶段(最大分蘖期至软面团阶段)进行 BPH 抗性表型分析将有助于提高 QTL 检测效率和这些 QTL 向优良水稻品种的有效渗入,这是开发稳定抗性的重要途径。 BPH 和了解水稻对 BPH 的数量抗性。在检测到的 4 个 QTL 中,QBph6.1 (RM7158-RM19606)、QBph6.3 (RM402-RM276) 和 QBph12.1 (RM28378-RM28427) 与高表型变异和紧密连锁的标记相关,这使得它们在标记辅助选择中具有实际效用。结果表明,在作物生长敏感阶段(最大分蘖期至软面团阶段)进行 BPH 抗性表型分析将有助于提高 QTL 检测效率和这些 QTL 向优良水稻品种的有效渗入,这是开发稳定抗性的重要途径。 BPH 和了解水稻对 BPH 的数量抗性。在检测到的 4 个 QTL 中,QBph6.1 (RM7158-RM19606)、QBph6.3 (RM402-RM276) 和 QBph12.1 (RM28378-RM28427) 与高表型变异和紧密连锁的标记相关,这使得它们在标记辅助选择中具有实际效用。结果表明,在作物生长敏感阶段(最大分蘖期至软面团阶段)进行 BPH 抗性表型分析将有助于提高 QTL 检测效率和这些 QTL 向优良水稻品种的有效渗入,这是开发稳定抗性的重要途径。 BPH 和了解水稻对 BPH 的数量抗性。
更新日期:2020-06-28
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