Analytical prospects of ionic liquids as the components of the microemulsion for polyphenols separation by microemulsion electrokinetic chromatography are explored. Imidazolium-based hydrophilic ionic liquid 1-hexadecyl-3-methylimidazolium chloride was used as a surfactant and hydrophobic ionic liquids 1-hexyl-3-methylimidazolium tetrafluoroborate and 1-hexyl-3-methylimidazolium bis(trifluormethylsulfonyl)imide were used as the oil phase. It was shown that the addition of ionic liquids into the microemulsion leads to a significant increase in the number of theoretical plates for catechins separation (in the range 200–650 ×103) in both cases. It was established that the use of hydrophobic ionic liquids 1-hexyl-3-methylimidazolium tetrafluoroborate and 1-hexyl-3-methylimidazolium bis(trifluormethylsulfonyl)imide increases the sensitivity and selectivity of the polyphenols separation compared to 1-hexadecyl-3-methylimidazolium chloride. The following composition of microemulsion was found to be optimal for the selective electrophoretic separation of catechins with ionic liquids as the oil phase: 0.6% (w/v) sodium dodecyl sulphate, 0.7% (w/v) 1-hexyl-3-methylimidazolium tetrafluoroborate, 1.6% (w/v) 1-butanol, 5 mM phosphate buffer solution, pH = 7. The conditions for the electrophoretic separation of catechins with microemulsion electrokinetic chromatography were used to determine polyphenols in tea samples.

中文翻译：

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微乳液电动色谱中的咪唑鎓离子液体用于分离多酚抗氧化剂

探讨了离子液体作为微乳液组分用于微乳液电动色谱分离多酚的分析前景。咪唑基亲水离子液体1-十六烷基-3-甲基咪唑氯化物用作表面活性剂，疏水离子液体1-己基-3-甲基咪唑四氟硼酸盐和1-己基-3-甲基咪唑鎓双（三氟甲基磺酰基）亚胺用作油相. 结果表明，在两种情况下，将离子液体添加到微乳液中都会导致儿茶素分离的理论塔板数显着增加（范围为 200-650 × 103）。已确定，与 1-十六烷基-3-甲基咪唑氯化物相比，使用疏水性离子液体 1-己基-3-甲基咪唑鎓四氟硼酸盐和 1-己基-3-甲基咪唑鎓双（三氟甲基磺酰基）亚胺提高了多酚分离的灵敏度和选择性. 发现以下微乳液的组成对于以离子液体为油相的儿茶素的选择性电泳分离是最佳的：0.6% (w/v) 十二烷基硫酸钠，0.7% (w/v) 1-己基-3-甲基咪唑鎓四氟硼酸盐，1.6% (w/v) 1-丁醇，5 mM 磷酸盐缓冲溶液，pH = 7。使用微乳液电动色谱法对儿茶素进行电泳分离的条件用于测定茶样品中的多酚。