Currently, cognitive enhancers are considered necessary because they play a critical role in daily and social behaviors. The cognitive-enhancing effect of mulberry milk has gained attention due to the cognitive-enhancing effect of this anthocyanin-rich substance and the cognitive-enhancing effect of mulberry fruit in animal models. However, the effect of anthocyanin-rich mulberry milk in clinical trials especially in children is still unknown. This study was a randomized double-blind crossover intervention. A total of forty-six healthy, normal, cognitive subjects aged 6–12 years old were provided mulberry milk (containing mulberry 10 g) or placebo milk (50 mL). Attention and cognitive function were assessed using the auditory odd ball paradigm of event-related potential, whereas working memory was assessed using a computerized battery test. The assessment was performed at baseline and then at 1.5 and 3 hours postdosing. At the end of study period, the activities of acetylcholinesterase (AChE) and monoamine oxidase (MAO) together with that of saliva cortisol were determined. Following mulberry milk intervention, the decreased N100 latency and the increased P300 amplitude were increased both at 1.5 and 3 hours after dosing. The decreased response time of digit updating was observed both at 1.5 and 3 hours after dosing, whereas the decreased response time of picture updating was observed at 3 hours after dosing. In addition, the reduction of saliva cortisol was also observed at both periods. The improvement of attention and cognitive processing capabilities together with the working memory suggests the cognitive-enhancing potential of mulberry milk for school-age children. The possible underlying mechanism may be associated partly with the reduction of cortisol, a stress hormone.

中文翻译：

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单次服用桑Milk牛奶对6-12岁儿童认知功能的影响：一项来自随机，安慰剂对照，交叉研究的结果。

当前，认知增强剂被认为是必要的，因为它们在日常和社交行为中起着至关重要的作用。由于这种富含花青素的物质的认知增强作用和桑树果实在动物模型中的认知增强作用，桑树乳的认知增强作用受到关注。然而，在临床试验中，尤其是在儿童中，富含花青素的桑milk牛奶的效果仍然未知。这项研究是随机双盲交叉干预。总共向46名6-12岁的健康，正常，认知受试者提供了桑milk牛奶（含桑10 10克）或安慰剂奶（50毫升）。使用事件相关电位的听觉奇异球范式评估注意力和认知功能，而使用计算机电池测试评估工作记忆。评估在基线进行，然后在给药后1.5和3小时进行。在研究期末，测定乙酰胆碱酯酶（AChE）和单胺氧化酶（MAO）以及唾液皮质醇的活性。桑milk牛奶干预后，给药后1.5小时和3小时，N100潜伏期缩短，P300振幅增加。在给药后1.5和3小时观察到手指更新的响应时间减少，而在给药后3小时观察到图像更新的响应时间减少。此外，两个时期的唾液皮质醇水平均降低。注意力和认知加工能力以及工作记忆的提高表明，桑milk牛奶对于学龄儿童具有认知增强的潜力。