Introduction. Diabetes is increasingly prevalent at global level and associated with various impacts including the male reproductive system. Aims. This research is aimed at investigating the influence of diabetes on the localization and expression of HSP90 and iNOS in the testicular tissue of diabetic rats. Methods. A diabetic model was developed through a single injection of alloxan monohydrate intraperitoneally (purchased from Sigma-Aldrich) 120 mg/kg body weight following fasting for 12 hrs. The experiment involved two groups, the control and diabetic groups with 10 albino rats in each group. Diabetes was considered if glucose concentration was ≥200 mg/dl. The experiment duration was for one month. After the experiment had finished, all rats were terminated and prepared for routine histological and immunohistochemical examination. Results. The results revealed that diabetes caused morphological changes at histological level in testicular tissue. Immunohistochemical examination showed that diabetes significantly upregulated the expression of both HSP90 and iNOS in the testicular tissue of diabetic rats as compared with that of the control group (). Conclusion. Diabetes may induce adverse health effects on the male reproduction through upregulation of HSP90 and iNOS in the testicular tissue of diabetic rats.

中文翻译：

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HSP90 和 INOS 在糖尿病大鼠睾丸中的复合表达作为糖尿病的细胞和病理不良反应。

简介。糖尿病在全球范围内越来越普遍，并与包括男性生殖系统在内的各种影响有关。目标。本研究旨在探讨糖尿病对糖尿病大鼠睾丸组织中HSP90和iNOS定位和表达的影响。方法. 通过在禁食 12 小时后腹膜内单次注射四氧嘧啶一水合物（购自 Sigma-Aldrich）120 mg/kg 体重来开发糖尿病模型。实验涉及两组，对照组和糖尿病组，每组10只白化大鼠。如果葡萄糖浓度≥200 mg/dl，则考虑糖尿病。实验时间为一个月。实验结束后，处死所有大鼠，准备进行常规组织学和免疫组化检查。结果. 结果表明，糖尿病引起睾丸组织组织学水平的形态学变化。免疫组化检查显示，与对照组相比，糖尿病大鼠睾丸组织中HSP90和iNOS的表达均显着上调。）。 结论。糖尿病可能通过上调糖尿病大鼠睾丸组织中的 HSP90 和 iNOS 对雄性生殖造成不利的健康影响。