This study investigated the genetic basis of multidrug resistance in two strains of Achromobacter xylosoxidans isolated from patients attending a hospital in Thailand in 2012. These isolates were highly resistant to cephalosporins, aminoglycosides, fluoroquinolones, co-trimoxazole and carbapenems. Whole genome sequencing revealed that the two isolates were not clonally related and identified a carbapenem resistance gene-habouring integron (In687), residing in a novel genomic island, AcGI1. This In687 shares 100% identical nucleotide sequence with ones found in Acinetobacter baumannii Aci 16, isolated from the same hospital in 2007. We report the first analysis of multidrug-resistant A. xylosoxidans isolated in Thailand, and the first example of this island in A. xylosoxidans. Our data support the idea that resistance has spread in Thailand via horizontal gene transfer between species and suggest the possibility of A. xylosoxidans may serve as a reservoir of antibiotic resistance, especially in hospital setting.

中文翻译：

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AcGI1，一个新的基因组岛，在泰国教学医院的多重耐药木糖氧化无色杆菌中携带抗生素抗性整合子 In687。

本研究调查了2012 年从泰国一家医院就诊的患者中分离出的两种木糖氧化无色杆菌菌株多药耐药的遗传基础。这些菌株对头孢菌素、氨基糖苷类、氟喹诺酮类、复方新诺明和碳青霉烯类药物具有高度耐药性。全基因组测序显示，这两个分离株没有克隆相关性，并确定了一个碳青霉烯类抗性基因整合子 (In687)，位于一个新的基因组岛 AcGI1。该 In687 与2007 年从同一家医院分离的鲍曼不动杆菌Aci 16 中发现的核苷酸序列具有 100% 相同的核苷酸序列。我们报告了对多重耐药木糖氧化酶的首次分析在泰国隔离，并且在A. xylosoxidans 中该岛的第一个例子。我们的数据支持抗药性已通过物种之间的水平基因转移在泰国传播的观点，并表明木糖氧化酶 A. xylosoxidans可能充当抗生素抗药性的储存库，尤其是在医院环境中。