Egyptian henbane (Hyoscyamus muticus L.) plants are rich sources of alkaloids used in pharmaceutical products. Recently, rising efforts have been devoted to reducing mineral fertilizer supply, production cost, and environmental pollution via decreasing the doses of nitrogenous fertilizers and adopting biofertilizer farming systems. Two field experiments were conducted to examine the potential role of N fixing bacteria Azotobacter spp. and Azospirillum spp. on the growth, mineral status, tropane alkaloids, leaf anatomy, and seed yield of Egyptian henbane grown with different levels of mineral nitrogen fertilizer, i.e., 25%, 50%, and 100% of the recommended dose, equal to 30, 60, and 120 kg N ha⁻¹. N fertilizer improved growth, mineral elements, tropane alkaloids, seed yield, and yield components of Egyptian henbane, which showed a gradually rising trend as the rate of N fertilizer increased. High doses of N fertilizer presumably elicited favorable changes in the anatomical structure of Egyptian henbane leaves. The application of 50% N dose plus N fixing bacteria affected Egyptian henbane trials similarly to 100% of recommended N dose. In conclusion, the N fixing bacteria proved to be a sustainable tool for a two-fold reduction in the recommended dose of mineral N fertilizer and the sustainable management of Egyptian henbane nutrition.

中文翻译：

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固氮细菌—不同矿质氮营养条件下埃及Hen的生长和化学组成的有效促进剂

埃及henbane（Hyoscyamus muticus L.）植物是药品中使用的生物碱的丰富来源。近来，通过减少氮肥的剂量和采用生物肥料耕作系统，致力于减少矿物肥料的供应，生产成本和环境污染的不断努力。进行了两个野外实验，以检验固氮细菌固氮菌的潜在作用。和固氮螺菌属。不同浓度的矿物氮肥（即建议剂量的25％，50％和100％，等于30、60）下生长的埃及b的生长，矿物质状态，托烷生物碱，叶片解剖结构和种子产量，和120 kg N ha ^-1。氮肥改善了埃及hen的生长，矿质元素，托烷生物碱，种子产量和产量构成，并随着氮肥用量的增加而呈逐渐上升的趋势。推测高剂量的氮肥会引起埃及Egyptian树叶片的解剖结构发生有利变化。50％N剂量加N固定细菌的施用对埃及henbane试验的影响与100％推荐N剂量相似。总而言之，固氮菌被证明是将矿质氮肥的推荐剂量减少两倍并可持续管理埃及山b营养的一种可持续工具。