Vitamin D measurements in biological fluids by liquid chromatography–tandem mass spectrometry (LC–MS/MS) have been widely used but remain challenging at very low concentration levels. Rapid, high recovery, sensitive and reliable measurements of vitamin D, as well as its primary metabolites using LC–MS/MS are urgently needed for a routine clinical laboratory. Herein, we reported a novel electrospray LC–MS/MS method for determining vitamin D and its primary metabolites using the supported liquid extraction method to achieve higher recoveries, with optimized pH values to achieve optimal derivatization efficiency for higher sensitivity and selected chromatographic conditions to shorten the separation time. The method has been validated with respect to selectivity, recovery, matrix effects, accuracy and precision, stabilities, carryover and dilution effects. The method has been successfully applied to quantify the VD plasma concentrations of depressive, schizophrenic patients and healthy individuals. The result showed that there were significant differences in plasma VD levels between mental disorder patients with healthy individuals, and the total VD levels in mental disorder patients were much higher than healthy individuals, which might require larger clinical samples for validation.

中文翻译：

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使用电喷雾LC-MS / MS同时测定人血浆中的VD2，VD3、25（OH）D2和25（OH）D3及其在评估抑郁症，精神分裂症患者和健康个体中的VD血浆水平中的应用。

通过液相色谱-串联质谱法（LC-MS / MS）测定生物液中的维生素D已被广泛使用，但在极低的浓度下仍具有挑战性。常规临床实验室迫切需要使用LC-MS / MS对维生素D及其主要代谢产物进行快速，高回收率，灵敏和可靠的测量。本文中，我们报道了一种新型的电喷雾LC-MS / MS方法，该方法使用支持的液体萃取方法来测定维生素D及其主要代谢物，以实现更高的回收率，并优化了pH值以实现最佳的衍生化效率，从而获得了更高的灵敏度，并缩短了所选的色谱条件分离时间。该方法在选择性，回收率，基质效应，准确性和精密度，稳定性，残留和稀释效应。该方法已成功应用于量化抑郁症，精神分裂症患者和健康个体的VD血浆浓度。结果表明，精神障碍患者与健康个体之间血浆VD水平存在显着差异，精神障碍患者的总VD水平远高于健康个体，这可能需要更大的临床样本进行验证。