Strain 28bB2T^T is a sulfate-reducing bacterium isolated in a previous study, obtained from a p-xylene-degrading enrichment culture. Physiological, phylogenetic and genomic characterizations of strain 28bB2T^T were performed to establish the taxonomic status of the strain. Cells of strain 28bB2T^T were short oval-shaped (0.8–1.2 × 1.2–2.7 μm), motile, and Gram-negative. For growth, the optimum pH was pH 6.5–7.0 and the optimum temperature was 28–32 °C. Strain 28bB2T^T oxidized toluene but could not utilize p-xylene. Sulfate and thiosulfate were used as electron acceptors. The G + C content of the genomic DNA was 53.8 mol%. The genome consisted of an approximately 8.3 Mb of chromosome and two extrachromosomal elements. On the basis of 16S rRNA gene analysis, strain 28bB2T^T was revealed to belong to the genus Desulfosarcina, with high sequence identities to Desulfosarcina ovata oXyS1^T (99.5%) and Desulfosarcina cetonica DSM 7267^T (98.7%). Results of Average Nucleotide Identity (ANI) calculation and digital DNA–DNA hybridization (dDDH) analysis showed that the strain 28bB2T^T should be classified as a subspecies under D. ovata. Based on physiological and phylogenetic data, strain 28bB2T^T (=NBRC 106234 =DSM 23484) is proposed as the type strain of a novel species in genus Desulfosarcina, Desulfosarcina ovata subsp. sediminis subsp. nov.

菌株28bB2T ^T是在先前的研究中分离的硫酸盐还原细菌，得自对二甲苯降解的浓缩培养物。进行了菌株28bB2T ^T的生理，系统发育和基因组鉴定，以建立菌株的分类学状态。菌株28bB2T ^T的细胞为短椭圆形（0.8–1.2×1.2–2.7μm），运动型和革兰氏阴性。为了生长，最适pH为pH 6.5-7.0，最适温度为28-32°C。菌株28bB2T ^T氧化甲苯但不能利用p-二甲苯。硫酸盐和硫代硫酸盐用作电子受体。基因组DNA的G + C含量为53.8mol％。该基因组由大约8.3 Mb的染色体和两个染色体外元件组成。在16S rRNA基因分析的基础上，发现菌株28bB2T ^T属于脱硫弧菌属，与卵形脱硫弧菌oXyS1 ^T（99.5％）和Cetonica DSM 7267 ^T（98.7％）具有高度的序列同一性。平均核苷酸同一性（ANI）计算结果和数字DNA-DNA杂交（dDDH）分析结果表明，应将28bB2T ^T菌株归类为卵形线虫的一个亚种。。基于生理和系统发育数据，菌株28bB2T ^Ť（= NBRC 106234 = DSM 23484）被提出作为在属一个新种的模式菌株Desulfosarcina，Desulfosarcina卵形亚种。塞迪米尼斯亚种 十一月