By using BPMOH as the fluorophore and 2, 4-dinitrobenzenesulfonate moiety as the recognition site for thiols, a new long-wavelength emission (645 nm) fluorescent probe BPMSH with large Stokes shift (133 nm) was designed and synthesized. Probe BPMSH exhibited almost no fluorescence emission because of the PET process. When adding thiols, BPMSH could be quickly converted into BPMOH emitting a significant red fluorescence at 645 nm. In addition, BPMSH displayed high selectivity toward thiols among various biologically related analytes. Probe BPMSH has been applied to exogenous and endogenous thiols detection and imaging in living MCF-7 cells and MGC-803 cells. Most importantly, this probe BPMSH was successfully utilized for imaging thiols in zebrafish.

以BPMOH为荧光团，以2-二硝基苯磺酸盐部分为硫醇的识别位点，设计合成了斯托克斯位移大（133 nm）的新型长波长发射（645 nm）荧光探针BPMSH。由于PET过程，探针BPMSH几乎没有荧光发射。当添加硫醇时，BPMSH可以迅速转化为BPMOH，在645 nm处发出明显的红色荧光。另外，BPMSH在各种生物相关分析物中显示出对硫醇的高选择性。探针BPMSH已应用于活的MCF-7细胞和MGC-803细胞的外源和内源硫醇检测和成像。最重要的是，该探针BPMSH已成功用于斑马鱼中的硫醇成像。