β-Conglycinin is an important storage protein in soybean, which can potentially cause food allergies in human. In this study, a sensitive mouse monoclonal antibody (3D11 mAb) with a high affinity was prepared, and sandwich lateral flow immunochromatographic detection strips were developed for the rapid detections of the soybean allergen β-conglycinin. The 3D11 mAb was combined with a rabbit polyclonal antibody in order to establish strips. The titer of 3D11 mAb was 1:2.56 × 10⁵. The affinity constant of the 3D11 mAb was 9.6 × 10⁹. The lowest detection limit with the naked eye of the double antibody sandwich strips was 1 μg/mL. In addition, chemical molecules p-aminothiophenol with colloidal gold were used as Raman enhancement signals in order to achieve quantitative detections of the β-conglycinin. It was determined in this study that the practical working range of the β-conglycinin concentrations was between 160 ng/mL and 100 μg/mL with the developed assay.

β-伴大豆球蛋白是大豆中重要的储存蛋白，可能引起人类食物过敏。本研究制备了高亲和力的灵敏小鼠单克隆抗体（3D11 mAb），并开发了夹心侧流免疫层析检测试纸条，用于快速检测大豆过敏原β-伴大豆球蛋白。将 3D11 mAb 与兔多克隆抗体结合以建立条带。 3D11 mAb的滴度为1:2.56×10 ⁵ 。 3D11 mAb的亲和常数为9.6×10 ⁹ 。双抗体夹心条肉眼检测最低限为1 μg/mL。此外，利用胶体金化学分子对氨基苯硫酚作为拉曼增强信号，实现了β-伴大豆球蛋白的定量检测。本研究确定，所开发的测定法的 β-伴大豆球蛋白浓度的实际工作范围在 160 ng/mL 至 100 μg/mL 之间。