Diclofenac (DCF) and ibuprofen (IBU) are widely used anti-inflammatory drugs and are frequently detected in wastewater from Wastewater Treatment Plants and in aquatic environments. In this study, the methanogenic potential (P) of anaerobic sludge subjected to DCF (7.11 ± 0.02 to 44.41 ± 0.05 mg L⁻¹) and IBU (6.11 ± 0.01 to 42.61 ± 0.05 mg L⁻¹), in sanitary sewage, was investigated in batch reactors. Cosubstrates (200 mg L⁻¹ of organic matter) in the form of ethanol, methanol:ethanol and fumarate were tested separately for the removal of drugs. In the DCF assays, P was 6943 ± 121 μmolCH₄, 9379 ± 259 μmolCH_4, 9897 ± 212 μmolCH₄ and 11,530 ± 368 μmolCH₄ for control, fumarate, methanol:ethanol and ethanol conditions, respectively. In the IBU assays, under the same conditions, P was 6145 ± 101 μmolCH₄, 6947 ± 66 μmolCH₄, 8141 ± 191 μmolCH₄and 10,583 ± 512 μmolCH₄, respectively. Without cosubstrates, drug removal was below 18% for 43.10 ± 0.01 mgDCF L⁻¹ and 43.12 ± 0.03 mgIBU L⁻¹, respectively. Higher P and removal of DCF (28.24 ± 1.10%) and IBU (18.72 ± 1.60%) with ethanol was observed for 43.20 ± 0.01 mgDCF L⁻¹ and 43.42 ± 0.03 mgIBU L⁻¹, respectively. This aspect was better evidenced with DCF due to its molecular structure, a condition that resulted in a higher diversity of bacterial populations. Through the 16S rRNA sequencing, bacteria genera capable of performing aromatic ring cleavage, β-oxidation and oxidation of ethanol and fatty acids were identified. Higher relative abundance (>0.6%) was observed for Smithella, Sulfuricurvum and Synthophus for the Bacteria Domain and Methanosaeta (>79%) for the Archaea Domain. The use of ethanol favored greater mineralization of organic matter and greater methane production, which can directly assist in the metabolic pathways of microorganisms.

双氯芬酸（DCF）和布洛芬（IBU）是广泛使用的消炎药，经常在废水处理厂的废水和水生环境中检测到。在这项研究中，厌氧污泥在生活污水中经受DCF（7.11±0.02至44.41±0.05 mg L ^-1）和IBU（6.11±0.01至42.61±0.05 mg L ^-1）的产甲烷潜力为（P）在间歇反应器中进行了研究。分别测试了乙醇，甲醇：乙醇和富马酸酯形式的共底物（200 mg L ^-1的有机物）对药物的去除。在DCF测定中，P为6943±121μmolCH ₄，9379±259μmolCH _4， 9897±212μmolCH ₄和11530±368μmolCH ₄用于控制，富马酸盐，甲醇：乙醇分别和乙醇的条件。在IBU测定中，在相同条件下，P为6145±101μmolCH ₄，6947±66μmolCH ₄，8141±191μmolCH ₄和10583±512μmolCH ₄分别。在没有共底物的情况下，对于43.10±0.01 mgDCF L ^-1和43.12±0.03 mgIBU L ^-1，药物去除率分别低于18％。对于43.20±0.01 mgDCF L ^-1和43.42±0.03 mgIBU L ^-1，观察到更高的P以及用乙醇去除DCF（28.24±1.10％）和IBU（18.72±1.60％）， 分别。DCF由于其分子结构而更好地证明了这一方面，这种条件导致细菌种群的多样性更高。通过16S rRNA测序，鉴定出能够进行芳香环裂解，β-氧化以及乙醇和脂肪酸氧化的细菌属。（> 0.6％），观察到较高的相对丰度为Smithella，Sulfuricurvum和Synthophus为细菌域和Methanosaeta（> 79％）为古细菌域。乙醇的使用有利于有机物的更大矿化和甲烷的产生，这可以直接帮助微生物的代谢途径。