The underlying hypothesis of this study is that simple potentiometric measurements between sensing electrodes and a shared reference electrode - Microbial Potentiometric Sesnor (MPS) system - can be employed in a long-term, continuous mode of operation to resolve the spatial and temporal changes in environmental systems. To address the hypothesis, (1) a conceptual description of the MPS system and its postulated principle of operation are provided; (2) the MPS system performance is documented under controlled laboratory conditions; and (3) the capabilities of the MPS system are documented under quiescent and dynamic field condition. In a laboratory setting, the variability among different MPS signals was insignificant confirming the postulated high accuracy and reproducibility of the sensor performance. It also demonstrated statistically significant correlations with dissolved oxygen (DO) and oxidation-reduction potential (ORP) sensors, while showing capabilities of operating under anoxic and anaerobic conditions. Regardless of their locations in the model wetland system, three MPS sensors functioned without interruption and cleaning for a period >2 years, and thus demonstrating long-term durability of the MPS technology. In real batch-wastewater treatment facility, the deployed MPS system signals were able to describe the organic carbon trends and correlate with each treatment phase in a cycle. Data reproducibility and reliability exceeded the expectations better describing the carbon treatment levels than the DO and ORP sensors (p < 4.4 × 10⁻¹⁶² vs phase adjusted p < 3.0 × 10⁻⁵⁸). While MPS signals correlate with specific parameters that describe the local process or environments, it is more prudent to employ both the magnitude and pattern of a composite signal like the MPS signal describe the change to reflect any shift in the local environment. When compared to a baseline pattern, this change in signal magnitude and pattern reveals important information that can be employed to tailor and optimize any condition or process which involves microorganisms.

该研究的基本假设是，可以长期，连续地使用操作电极和共享参比电极之间的简单电位测量-微生物电位Sesnor（MPS）系统，以解决环境的时空变化。系统。为了解决该假设，（1）提供了MPS系统的概念描述及其假定的工作原理；（2）在受控实验室条件下记录MPS系统性能；（3）在静态和动态现场条件下记录了MPS系统的功能。在实验室环境中，不同MPS信号之间的差异很小，这证实了传感器性能的假定高精度和可重复性。它还显示了与溶解氧（DO）和氧化还原电位（ORP）传感器的统计显着相关性，同时显示了在缺氧和厌氧条件下的操作能力。不管它们在模型湿地系统中的位置如何，三个MPS传感器在不中断和清洁的情况下都能运行超过2年，因此证明了MPS技术的长期耐用性。在实际的分批废水处理设施中，部署的MPS系统信号能够描述有机碳趋势，并与循环中的每个处理阶段相关。与DO和ORP传感器相比，数据的可重复性和可靠性超出了预期，更好地描述了碳处理水平（同时显示在缺氧和厌氧条件下的操作能力。不管它们在模型湿地系统中的位置如何，三个MPS传感器在不中断和清洁的情况下都能运行超过2年，因此证明了MPS技术的长期耐用性。在实际的分批废水处理设施中，部署的MPS系统信号能够描述有机碳趋势，并与循环中的每个处理阶段相关。与DO和ORP传感器相比，数据的可重复性和可靠性超出了预期，更好地描述了碳处理水平（同时显示在缺氧和厌氧条件下的操作能力。不管它们在模型湿地系统中的位置如何，三个MPS传感器在不中断和清洁的情况下都能运行超过2年，因此证明了MPS技术的长期耐用性。在实际的分批废水处理设施中，部署的MPS系统信号能够描述有机碳趋势，并与循环中的每个处理阶段相关。与DO和ORP传感器相比，数据的可重复性和可靠性超出了预期，更好地描述了碳处理水平（因此证明了MPS技术的长期耐用性。在实际的分批废水处理设施中，部署的MPS系统信号能够描述有机碳趋势，并与循环中的每个处理阶段相关。与DO和ORP传感器相比，数据的可重复性和可靠性超出了预期，更好地描述了碳处理水平（因此证明了MPS技术的长期耐用性。在实际的分批废水处理设施中，部署的MPS系统信号能够描述有机碳趋势，并与循环中的每个处理阶段相关。与DO和ORP传感器相比，数据的可重复性和可靠性超出了预期，更好地描述了碳处理水平（p  <4.4×10 ^-162与相位调整后的p  <3.0×10 ^-58）。尽管MPS信号与描述局部过程或环境的特定参数相关，但更谨慎地使用复合信号的幅度和模式，就像MPS信号描述变化以反映局部环境的任何变化一样。当与基线模式进行比较时，信号大小和模式的这种变化揭示了重要信息，可用于调整和优化涉及微生物的任何条件或过程。