Lipidomic analysis of diatoms cultivated with silica nanoparticles,Phytochemistry

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Lipidomic analysis of diatoms cultivated with silica nanoparticles
Phytochemistry ( IF 3.2 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.phytochem.2020.112452
Tomáš Řezanka ₁ , Michal Řezanka ₂ , Dana Mezricky ₃ , Milada Vítová ₄

Affiliation

Polar lipids from the diatoms Diadesmis gallica and Navicula atomus were separated and their structures were determined using high resolution tandem MS HILIC-LC/ESI. This method allowed us to identify 34 classes of lipids, each containing dozens of molecular species, including regioisomers. The largest differences were found in two sulfur-containing lipids, sulfoquinovosyldiacylglycerol and phosphatidylsulfocholine caused probably by the remodeling of lipid species. These diatoms have been found to use several mechanisms to resolve growth in extreme environments, i.e. silica starvation. The presence of insoluble nano-SiO2 leads to the replacement of cellular phospholipids with sulfolipids. Regioisomer ratios also vary depending on the concentration of nano-SiO2 in the culture medium, i.e. the biosynthesis of polar lipids via the prokaryotic (plastidial) and/or eukaryotic (explastidial) pathways. Complex analyses of polar lipids using high resolution HILIC-LC/ESI-tandem, as used for diatoms, can also be used for other photosynthetic microorganisms.

中文翻译：

用二氧化硅纳米粒子培养的硅藻的脂质组学分析

分离来自硅藻 Diadesmis gallica 和 Navicula atomus 的极性脂质，并使用高分辨率串联 MS HILIC-LC/ESI 确定其结构。这种方法使我们能够识别 34 类脂质，每类都包含数十种分子种类，包括区域异构体。在两种含硫脂质中发现了最大的差异，磺基奎诺糖基二酰基甘油和磷脂酰磺胆碱可能是由脂质种类的重塑引起的。已发现这些硅藻使用多种机制来解决极端环境中的生长问题，即二氧化硅饥饿。不溶性纳米 SiO2 的存在导致细胞磷脂被硫脂取代。区域异构体比率还取决于培养基中纳米 SiO2 的浓度，即通过原核（质体）和/或真核（质体）途径生物合成极性脂质。使用高分辨率 HILIC-LC/ESI 串联对极性脂质进行复杂分析，如用于硅藻，也可用于其他光合微生物。

更新日期：2020-09-01

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