Knockdown of LmCYP303A1 alters cuticular hydrocarbon profiles and increases the susceptibility to desiccation and insecticides in Locusta migratoria,Pesticide Biochemistry and Physiology

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Knockdown of LmCYP303A1 alters cuticular hydrocarbon profiles and increases the susceptibility to desiccation and insecticides in Locusta migratoria
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.pestbp.2020.104637
Lixian Wu ₁ , Zhitao Yu ₂ , Qiangqiang Jia ₃ , Xueyao Zhang ₂ , Enbo Ma ₂ , Sheng Li ₃ , Kun Yan Zhu ₄ , René Feyereisen ₅ , Jianzhen Zhang ₂

Affiliation

Cytochrome P450 monooxygenases (CYPs) serve many functions in insects, from the regulation of development to xenobiotic detoxification. Several conserved CYPs have been shown to play a role in insect growth and development. CYP303A1 is a highly conserved CYP with a single ortholog in most insects, but its underlying molecular characteristics and specific physiological functions remain poorly understood. In Drosophila melanogaster and Locusta migratoria, CYP303A1 is indispensable for eclosion to adult. Here, we report additional functions of the locust gene LmCYP303A1 in nymphal molts, cuticular lipid deposition and insecticide penetration. RT-qPCR revealed that LmCYP303A1 had a high expression level before ecdysis and was highly expressed in integument, wing pads, foregut and hindgut. Suppression of LmCYP303A1 expression by RNA interference (RNAi) caused a lethal phenotype with molting defect from nymph to nymph. In addition, LmCYP303A1 RNAi resulted in locusts being more susceptible to desiccation and to insecticide toxicity. Furthermore, knockdown of LmCYP303A1 efficiently suppressed the transcript level of key genes (ELO7, FAR15 and CYP4G102) responsible for cuticular hydrocarbon (CHC) synthesis, which led to a decrease in some CHC levels. Taken together, our results suggest that one of the functions of LmCYP303A1 is to regulate the biosynthesis of CHC, which plays critical roles in protecting locusts from water loss and insecticide penetration.

中文翻译：

LmCYP303A1 的敲低改变了角质层的碳氢化合物谱，并增加了蝗虫对干燥和杀虫剂的敏感性

细胞色素 P450 单加氧酶 (CYPs) 在昆虫中具有许多功能，从发育调节到异生物质解毒。几种保守的 CYPs 已被证明在昆虫生长和发育中发挥作用。CYP303A1 是一种高度保守的 CYP，在大多数昆虫中具有单个直向同源物，但其潜在的分子特征和特定的生理功能仍知之甚少。在 Drosophila melanogaster 和 Locusta migratoria 中，CYP303A1 对于羽化成虫是必不可少的。在这里，我们报告了蝗虫基因 LmCYP303A1 在若虫蜕皮、表皮脂质沉积和杀虫剂渗透中的其他功能。RT-qPCR 显示 LmCYP303A1 在蜕皮前具有高表达水平，并在外皮、翼垫、前肠和后肠中高表达。通过 RNA 干扰 (RNAi) 抑制 LmCYP303A1 表达导致致命表型，从若虫到若虫的蜕皮缺陷。此外，LmCYP303A1 RNAi 导致蝗虫对干燥和杀虫剂毒性更敏感。此外，敲低 LmCYP303A1 可有效抑制负责表皮碳氢化合物 (CHC) 合成的关键基因（ELO7、FAR15 和 CYP4G102）的转录水平，从而导致某些 CHC 水平降低。总之，我们的结果表明 LmCYP303A1 的功能之一是调节 CHC 的生物合成，这在保护蝗虫免受水分流失和杀虫剂渗透方面起着至关重要的作用。此外，敲低 LmCYP303A1 可有效抑制负责表皮碳氢化合物 (CHC) 合成的关键基因（ELO7、FAR15 和 CYP4G102）的转录水平，从而导致某些 CHC 水平降低。总之，我们的结果表明 LmCYP303A1 的功能之一是调节 CHC 的生物合成，这在保护蝗虫免受水分流失和杀虫剂渗透方面起着至关重要的作用。此外，敲低 LmCYP303A1 可有效抑制负责表皮碳氢化合物 (CHC) 合成的关键基因（ELO7、FAR15 和 CYP4G102）的转录水平，从而导致某些 CHC 水平降低。总之，我们的结果表明 LmCYP303A1 的功能之一是调节 CHC 的生物合成，这在保护蝗虫免受水分流失和杀虫剂渗透方面起着至关重要的作用。

更新日期：2020-09-01

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