Sporadic amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease for which there is no validated blood based biomarker. Extracellular vesicles (EVs) have the potential to solve this unmet clinical need. However, due to their heterogeneity and complex chemical composition, EVs are difficult to study. Raman spectroscopy (RS) is an optical method that seems particularly well suited to address this task. In fact, RS provides an overview of the biochemical composition of EVs quickly and virtually without any sample preparation. In this work, we studied by RS small extracellular vesicles (sEVs), large extracellular vesicles (lEVs) and blood plasma of sporadic ALS patients and of a matched cohort of healthy controls. The obtained results highlighted lEVs as a particularly promising biomarker for ALS. In fact, their Raman spectra show that sporadic ALS patients have a different lipid content and less intense bands relative to the aromatic amino acid phenylalanine.

偶发性肌萎缩性侧索硬化症（ALS）是一种神经退行性疾病，目前尚没有经过验证的基于血液的生物标记物。细胞外囊泡（EVs）有潜力解决这一未满足的临床需求。然而，由于它们的异质性和复杂的化学组成，电动汽车很难研究。拉曼光谱法（RS）是一种光学方法，似乎特别适合解决此任务。实际上，RS几乎不需要任何样品准备就可以快速而全面地了解电动汽车的生化成分。在这项工作中，我们通过RS研究了散发性ALS患者和健康对照人群的小细胞外囊泡（sEVs），大细胞外囊泡（lEVs）和血浆。获得的结果突出了lEV作为ALS的特别有前途的生物标记。事实上，