Verticillium dahliae is a significant pathogen in cucurbit cropping systems for which there are limited control options outside of soil fumigation. Endophytes, fungi and bacteria that live within plant hosts without impacting the host negatively, have exhibited antagonism to V. dahliae. The objectives of this study were to survey potential V. dahliae-antagonistic endophytes from roots of ‘Cinnamon Girl’ pumpkin (Cucurbita pepo) grown under either polyethylene (PE), an experimental polylactic acid/ poly(hydroxalkanoate) (PLA/PHA) mulch, Weed Guard Plus, or no mulch, as well as from ‘Sugar Baby’ watermelon (Citrullus lanatus), and ‘Tetsukabuto’ squash (C. maxima x C. moschata). Four selected endophytes were screened for antagonism against V. dahliae in the laboratory, greenhouse, and field.

A total of 777 isolates of potential fungal endophytes were recovered from pumpkin, watermelon, and squash roots between 2015 and 2016 of which 198 isolates were identified down to the genus level. Of those isolates, frequency of isolation was greatest for Dichotomopilus/Chaetomium spp. (5%), Cladosporium spp. (15.2 %), Clonostachys spp. (5.6 %), Epicoccum spp. (22.2 %), and Fusarium spp. (24.7 %). All five genera only weakly associated with roots grown under a particular mulch treatment (Cramer's V = 0.22) or cucurbit host (Cramer’s V = 0.1925). In a laboratory culture plate assay, V. dahliae isolate JAW-113 was plated against one of four prospective endophytes (Dichotomopilus sp., Epicoccum sp., Microdochium sp., or Schizothecium sp.). The area under the Verticillium culture growth curve (AUVGC) was significantly highest (P < 0.0001) when V. dahliae was by Schizothecium sp. or Dichotomopilus sp. In a greenhouse study using a Mason jar assay with V. dahliae amended potting mix, pumpkin plant vigor, plant fresh weight, root fresh weight, and root dry weight were significantly higher (P < 0.05) for plants inoculated with Dichotomopilus sp., Epicoccum sp., Microdochium sp., and Schizothecium sp. compared to plants without endophyte inoculation. Subsequent field trials in 2017 and 2018 showed no significant differences in foliar disease severity or fruit yield, regardless of whether plants were inoculated with an endophyte or not. However, recovery of V. dahliae colony forming units from pumpkin stem sap was significantly lower (P < 0.0001) for plants inoculated with either Dichotomopilus sp. or Schizothecium sp. in 2017 or Dichotomopilus sp. in 2018.

黄萎病菌是葫芦类作物种植系统中的重要病原体，在土壤熏蒸之外，其控制选择有限。生活在植物寄主中而不会对寄主造成负面影响的内生菌，真菌和细菌对大丽花弧菌表现出拮抗作用。这项研究的目的是调查在聚乙烯（PE），一种实验性聚乳酸/聚（羟基己酸酯）（PLA / PHA）覆盖膜下生长的'肉桂女孩'南瓜（Cucurbita pepo）的根中潜在的大丽花拮抗植物内生菌。，除草剂加或不加覆盖料，以及来自“糖宝贝”西瓜（Citrullus lanatus）和“ Tetsukabuto”南瓜（C. maxima x C. moschata））。在实验室，温室和田地中，筛选了四种选择的内生菌对大丽弧菌的拮抗作用。

在2015年至2016年之间，共从南瓜，西瓜和西葫芦根中回收了777株潜在真菌内生菌，其中198株分离到属下水平。在这些分离株中，Dichotomopilus / Chaetomium spp的分离频率最大。（5％），Cladosporium spp。（15.2％），梭菌属。（5.6％），淫羊。（22.2％）和镰刀菌属。（24.7％）。这五个属仅与在特定覆盖处理（克拉默氏V = 0.22）或葫芦科寄主（克拉默氏V = 0.1925）下生长的根弱相关。在实验室培养板分析中，大丽花弧菌将分离的JAW-113接种于四种预期内生菌之一（Dichotomopilus sp。，Epicoccum sp。，Microdochium sp。或Schizothecium sp。）中。黄萎病菌（Schizothecium sp ）对黄萎病菌生长曲线（AUVGC）的面积显着最高（P <0.0001）。或Dichotomopilus sp。在使用梅森罐测定法和大丽花苗修正的盆栽混合物进行的温室研究中，接种了H2O3的植物的南瓜植物活力，植物鲜重，根鲜重和根干重均显着更高（P <0.05）。Dichotomopilus sp。，Epicoccum sp。，Microdochium sp。和Schizothecium sp.。与没有内生菌接种的植物相比。随后的2017年和2018年的田间试验表明，无论是否给植物接种了内生菌，叶面病害严重程度或果实产量均无显着差异。然而，接种了双歧杆菌的植物从南瓜茎汁中回收大丽花菌的菌落形成单位明显较低（P <0.0001）。或Schizothecium sp。在2017年或Dichotomopilus sp。在2018年。