Abstract Sensitive and selective detection of nicotinamide adenine dinucleotide (NADH) is of great importance since it plays crucial roles in the living organism. In this work, a novel sandwich structural electrochemical sensor was fabricated by the electropolymerization of 3-aminophenylboronic acid (APBA) on reduced graphene oxide modified electrode for nicotinamide adenine dinucleotide (NADH) detection. Based on boronate affinity, NADH can be anchored on the sensing interface through the covalent bond between the cis-diol group of NADH and the boric acid of APBA. Moreover, due to the polyphenolic hydroxyl structure, the anchored NADH can further be bound with ferrocene boric acid (FcBA) in solution via the second boronate affinity. Thus, a dual-signal aroused from the oxidation peak currents of NADH and FcBA were employed for the detection of NADH with improved sensitivity, resulting in a wide linear range of 5.0 × 10−8 −1.0 × 10−5 mol/L and a low limit of detection of 2.6 × 10−8 mol/L (S/N = 3). In addition, double boronate affinity endowed the sensor with excellent selectivity to NADH. With the good repeatability and stability, the prepared sensor was employed to determine NADH in human blood serum samples with satisfactory results.

中文翻译：

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来自三明治结构传感接口的双信号用于 NADH 电化学灵敏检测

摘要 烟酰胺腺嘌呤二核苷酸 (NADH) 的灵敏和选择性检测非常重要，因为它在生物体中起着至关重要的作用。在这项工作中，通过 3-氨基苯基硼酸 (APBA) 在还原氧化石墨烯修饰电极上的电聚合制备了一种新型夹心结构电化学传感器，用于烟酰胺腺嘌呤二核苷酸 (NADH) 检测。基于硼酸盐的亲和力，NADH 可以通过 NADH 的顺式二醇基团和 APBA 的硼酸之间的共价键锚定在传感界面上。此外，由于多酚羟基结构，锚定的 NADH 可以通过第二硼酸盐亲和力进一步与溶液中的二茂铁硼酸 (FcBA) 结合。因此，NADH 和 FcBA 的氧化峰电流产生的双信号用于检测 NADH，提高了灵敏度，产生了 5.0 × 10-8 -1.0 × 10-5 mol/L 的宽线性范围和下限2.6 × 10−8 mol/L (S/N = 3) 的检测。此外，双硼酸盐亲和力赋予传感器对 NADH 优异的选择性。所制备的传感器具有良好的重复性和稳定性，用于测定人血清样品中的NADH，结果令人满意。