The aim of the study was to explore the antifungal and aflatoxin B₁ inhibitory efficacy of nanoencapsulated antifungal formulation. Mixture design response surface methodology (RSM) was utilized to design the antifungal formulation (SBC 4:1:1) based on the combination of chemically characterized Ocimum sanctum (S), O. basilicum (B), and O. canum (C) against Aspergillus flavus. The SBC was incorporated inside the chitosan nanomatrix (Ne-SBC) using an ultrasonic probe (40 kHz) and interactions were confirmed by SEM, FTIR and XRD analysis. The results showed that the Ne-SBC possessed enhanced antifungal and aflatoxin B₁ inhibitory effect over the free form of SBC. The biochemical and in silico results indicate that the antifungal and aflatoxin B₁ inhibitory effect was related to perturbance in the plasma membrane function (ergosterol biosynthesis and membrane cation) mitochondrial membrane potential, C-sources utilization, antioxidant defense system, and the targeted gene products Erg 28, cytochrome c oxidase subunit Va, and Nor-1. In-situ observation revealed that Ne-SBC effectively protects the Avena sativa seeds from A. flavus and AFB₁ contamination and preserves its sensory profile. The findings suggest that the fabrication of SBC inside the chitosan nano-matrix has promising use in the food industries as an antifungal agent.

这项研究的目的是探索纳米胶囊抗真菌制剂的抗真菌和黄曲霉毒素B ₁抑制功效。基于化学特征的圣殿（S），罗勒草（B.）和香菜（C.）的混合物，使用混合物设计响应面方法（RSM）设计抗真菌制剂（SBC 4：1：1）。对抗黄曲霉。使用超声探头（40 kHz）将SBC掺入壳聚糖纳米基质（Ne-SBC）内，并通过SEM，FTIR和XRD分析确认相互作用。结果表明，Ne-SBC具有较强的抗真菌和黄曲霉毒素B _{1的能力。}对SBC游离形式的抑制作用。生化和计算机模拟结果表明，抗真菌和黄曲霉毒素B ₁抑制作用与质膜功能（麦角固醇生物合成和膜阳离子）线粒体膜电位，碳源利用，抗氧化防御系统和目标基因产物的扰动有关。 Erg 28，细胞色素C氧化酶亚基Va和Nor-1。原位观察表明，Ne-SBC有效地保护了燕麦种子免受黄曲霉菌和AFB _1的侵害。污染并保留其感官特征。研究结果表明，在壳聚糖纳米基质内部制造SBC作为抗真菌剂在食品工业中具有广阔的应用前景。