There is a re-emerging interest in natural products as reputable sources of new active pharmaceutical ingredients. This study synchronously reports in vitro, with more than one cell line, and in vivo biological activities of extracts obtained from Sambucus nigra. Using several solvents and techniques, eighteen extracts were obtained from fresh and dried berries, and fresh flowers. The flavonoid content and identification were determined using HPLC-MS/MS. The extracts were then screened for antioxidant activity, total polyphenol content, collagenase, elastase, tyrosinase and acetylcholinesterase inhibition as well as photoprotection. In vitro and in vivo (murine model) anti-inflammatory activity and cytotoxicity (skin and monocytic cells) were also studied. The most promising extracts were those obtained from fresh flowers using either ultrasounds or methanol. These extracts showed similar results to positive controls, particularly the antioxidant activity (74.5 ± 1.6 %), collagenase inhibition (93.6 ± 0.6 %), photoprotection (Sun Protection Factor >50), in vitro anti-inflammatory activity (96.9 ± 2.9 %), as well as oral/topical anti-inflammatory activity. The ultrasounds/ethanol extract of fresh flowers presented higher collagenase inhibition (88.3 ± 2.8 %) and in vitro anti-inflammatory activity (101.8 ± 1.5 %). Cytotoxicity testing confirmed the safety. Chemical characterization allowed the deduction of a correlation between extract composition and biological activities, suggesting a straightforward application in the development of novel products subject to further investigation.

天然产品作为新的活性药物成分的信誉良好的来源正在重新兴起。这项研究同步报告了从一个以上的细胞系中提取自黑接骨木（Sambucus nigra）提取物的体外和体内生物学活性。使用几种溶剂和技术，从新鲜和干燥的浆果以及鲜花中获得了十八种提取物。使用HPLC-MS / MS测定类黄酮的含量和鉴定。然后筛选提取物的抗氧化剂活性，总多酚含量，胶原酶，弹性蛋白酶，酪氨酸酶和乙酰胆碱酯酶抑制作用以及光保护作用。体外和体内（鼠模型）还研究了抗炎活性和细胞毒性（皮肤和单核细胞）。最有希望的提取物是使用超声波或甲醇从鲜花中提取的提取物。这些提取物显示出与阳性对照相似的结果，特别是抗氧化剂活性（74.5±1.6％），胶原酶抑制（93.6±0.6％），光保护（防晒系数> 50），体外抗炎活性（96.9±2.9％）以及口服/局部抗炎活性。超声波/鲜花的乙醇提取物对胶原蛋白的抑制作用较高（88.3±2.8％），在体外抗炎活性（101.8±1.5％）。细胞毒性测试证实了安全性。化学表征可以推断出提取物的成分与生物学活性之间的相关性，这暗示了在需要进一步研究的新产品开发中的直接应用。