Malachite Green (MG) has a worldwide application in aquaculture as a therapeutic agent; however, its use in food producing animals is illegal, due to potential carcinogenicity and persistence of residues. This pilot study, the first conducted in Armenia, aimed to determine the concentration of MG residues in flesh of fish grown in artificial ponds of Armenia and conduct dietary exposure assessment to characterize possible health risks to consumers.

Detection of MG residues, including the major metabolite leucomalachite, was carried out in 29 fish composite samples by ELISA. The results were confirmed by LC-MS/MS. To determine fish consumption values, a food frequency questionnaire was used. Possible health risks were evaluated by calculating the Margin of Exposure (MOE) based on BMDL of 13 (neoplastic effects) and 6 (non-neoplastic effects) mg/kg bw.

In 34.5% of the investigated fish samples MG residues exceeded the minimum required performance limit. For BMDL₁₀ and BMDL_0.5, the MOEs ranged 3.36E+06–3.37E+07 and 1.55E+06–1.55E+07, respectively. The MOE for neoplastic effects was more than 10,000 and for non-neoplastic effects was more than 100. The results do not indicate public health concerns. However, the results highlight issues concerning the illegal use of MG in Armenian aquaculture, which deserves further attention.

孔雀石绿（MG）作为治疗剂已在水产养殖领域得到了广泛应用。然而，由于其潜在的致癌性和残留物的残留，将其用于食用动物是非法的。这项试验性研究是在亚美尼亚首次进行的，旨在确定亚美尼亚人工池塘中生长的鱼肉中MG残留的浓度，并进行饮食接触评估以表征对消费者的健康风险。

通过ELISA对29种鱼类复合样品中的MG残留物（包括主要代谢产物白孔雀石）进行检测。通过LC-MS / MS证实了结果。为了确定鱼的食用量，使用了食物频率调查表。通过根据BMDL为13（肿瘤效应）和6（非肿瘤效应）mg / kg bw来计算接触余量（MOE），评估可能的健康风险。

在34.5％的调查鱼类样品中，MG残留量超过了最低性能要求。对于BMDL ₁₀和BMDL _0.5，MOE分别为3.36E + 06–3.37E + 07和1.55E + 06–1.55E + 07。肿瘤效应的MOE大于10,000，非肿瘤效应的MOE大于100。结果未表明公共卫生问题。但是，结果突出了亚美尼亚水产养殖中非法使用MG的问题，值得进一步关注。