Abstract A novel technology named the freeze salting-out method was developed as an improvement to the traditional salting-out method, which requires a large amount of the salting-out agent and generates a large volume of high-salinity wastewater. Using the freeze salting-out method, the dosage of salting-out agent is only 5 wt%, compared to 20 wt% for the traditional approach. The electrical conductivity of the freeze salting-out effluent was 35.7 ms/cm, far lower than that generated by the traditional approach (330 ms/cm). Overall, 182 g of carmine with a high purity of 93.7% was produced from a 2 L crude carmine solution. The salting-out action enacted through freeze-concentration played a crucial role in the freeze salting-out process. The cost of carmine production using freeze salting-out technology was lower than that of the conventional salting-out method. Thus, this new method offers an alternative approach for extracting carmine and improving other salting-out processes.

中文翻译：

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冷冻盐析法降低胭脂红生产废水盐析剂用量和盐度的应用

摘要 冷冻盐析法是对需要大量盐析剂、产生大量高盐废水的传统盐析法的改进，开发了一种新技术——冷冻盐析法。使用冷冻盐析法，盐析剂的用量仅为5wt%，而传统方法为20wt%。冷冻盐析出水的电导率为35.7 ms/cm，远低于传统方法产生的电导率（330 ms/cm）。总体而言，从 2 L 粗胭脂红溶液中产生了 182 g 纯度为 93.7% 的高纯度胭脂红。通过冷冻浓缩进行的盐析作用在冷冻盐析过程中起着至关重要的作用。采用冷冻盐析技术生产胭脂红的成本低于常规盐析法。因此，这种新方法为提取胭脂红和改进其他盐析过程提供了一种替代方法。