Sepia pharaonis has great commercial value for aquaculture. However, it is sensitive to salinity fluctuations and lacking in genomic information. The present work utilized high-throughput transcriptome sequencing to assess the effect of low salinity (22.0 ppt) on gills of S. pharaonis. 6153 genes were identified as differentially expressed (p < 0.05), of which 3340 were increased and 2813 were decreased in low salinity group (22.0 ppt) relative to the control group (29.0 ppt). Subsequently, these DEGs were allocated to 226 KEGG pathways and 491 GO terms. Analysis of the transcriptome sequences and DEGs identified several unigenes and pathways involved in salt stress regulation. Moreover, the S. pharaonis carried 101,576 simple sequence repeats (SSRs). This is the first time osmoregulation in S. pharaonis has been explored by transcriptome sequencing. The data presented here reveals key insights into the genetic markers of salt stress in S. pharaonis.

法老棕褐色对水产养殖具有巨大的商业价值。但是，它对盐度波动和缺乏基因组信息敏感。本工作利用高通量转录组测序来评估低盐度（22.0 ppt）对法老葡萄球菌g的影响。 相对于对照组（29.0 ppt），低盐度组（22.0 ppt）鉴定出6153个差异表达基因（p <0.05），其中3340个基因表达增加而2813个基因表达减少。随后，将这些DEG分配给226个KEGG路径和491个GO项。转录组序列和DEG的分析确定了盐胁迫调控中涉及的几种单基因和途径。此外，法老葡萄球菌携带101,576个简单序列重复序列（SSR）。这是在第一时间渗透调节S.蚁已被转录组测序研究。这里提供的数据揭示了对法老葡萄盐胁迫遗传标记的重要见解。