Abstract The design of photobioreactors (PBRs) to treat municipal wastewater is in the early stages of development. Kinetics of activated sludge cannot be used for PBRs because biomass differs in composition, growth and decay rates. In this paper, respirometry was extended to a microalgal-bacterial consortium in a long-term PBR treating municipal wastewater. Kinetic and stoichiometric parameters (20 °C) were estimated for endogenous respiration, biodegradable COD oxidation and nitrification. The comprehensive respirometric approach was based on the Oxygen Production Rate (OPR) of photosynthetic microorganisms, and the Oxygen Uptake Rate (OUR) of the overall biomass, including data processing and corrections. The gross OPR (gOPR) was 9.8 ± 0.2 mg O2 g TSS−1 h−1; using microalgae quantified by flow cytometry, the per-cell gOPR was 11 fmol O2 cell−1 h−1. This oxygen was utilized for COD oxidation with rate of 18.0 and 19.3 mg g TSS−1 h−1 under light and dark, respectively. Biomass yield was 0.66 on average, similar to the typical values of activated sludge. The nitrification rate in the dark was 0.92 mg NH4+-N g TSS−1 h−1. The stoichiometric oxygen-over-ammonium coefficient was 4.4 g O2/g N under dark conditions (similar to activated sludge), but lower under light ones (2.8 g O2/g N). The respirometric approach proposed here added experimental kinetic results needed in the PBR design.

中文翻译：

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评估微藻-细菌群落处理实际城市废水的光合、异养和硝化活性的综合呼吸测量方法

摘要 用于处理城市污水的光生物反应器 (PBR) 的设计处于发展的早期阶段。活性污泥的动力学不能用于 PBR，因为生物质的组成、生长和衰减速率不同。在本文中，呼吸测量法扩展到长期 PBR 处理城市废水中的微藻-细菌聚生体。估计了内源呼吸、可生物降解 COD 氧化和硝化的动力学和化学计量参数 (20 °C)。综合呼吸测量方法基于光合微生物的产氧率 (OPR) 和整体生物质的吸氧率 (OUR)，包括数据处理和校正。总 OPR (gOPR) 为 9.8 ± 0.2 mg O2 g TSS-1 h-1；使用流式细胞术定量的微藻，每个细胞的 gOPR 为 11 fmol O2 cell−1 h−1。该氧气用于 COD 氧化，在光和暗下的速率分别为 18.0 和 19.3 mg g TSS-1 h-1。生物质产量平均为 0.66，与活性污泥的典型值相似。黑暗中的硝化率为 0.92 mg NH4+-N g TSS-1 h-1。在黑暗条件下（类似于活性污泥），化学计量的氧氨系数为 4.4 g O2/g N，但在光照条件下较低（2.8 g O2/g N）。这里提出的呼吸测量方法增加了 PBR 设计中所需的实验动力学结果。92 mg NH4+-N g TSS-1 h-1。在黑暗条件下（类似于活性污泥），化学计量的氧氨系数为 4.4 g O2/g N，但在光照条件下较低（2.8 g O2/g N）。这里提出的呼吸测量方法增加了 PBR 设计中所需的实验动力学结果。92 mg NH4+-N g TSS-1 h-1。在黑暗条件下（类似于活性污泥），化学计量的氧氨系数为 4.4 g O2/g N，但在光照条件下较低（2.8 g O2/g N）。这里提出的呼吸测量方法增加了 PBR 设计中所需的实验动力学结果。