This study aimed to examine the effects of algae (rich in omega-3 fatty acids), sunflower oil (rich in omega-6 fatty acids) and soybean oil (rich in omega-6 fatty acids) on the entire folliculogenesis in juvenile and sexually mature rabbits. After weaning, rabbits were randomly divided into four experimental groups of 14 animals each. Control animals received non-supplemented pellets, while in the other groups, the pellets contained 1% marine algae, 3% sunflower oil or 3% soybean oil. Animals from each group were slaughtered at 12 weeks of age (n = 7 per group) or at 18 weeks of age (n = 7 per group). The ovaries were harvested and fixed for hematoxylin-eosin staining, immunohistochemical localization of PCNA and TUNEL assay. Algae-enriched diet markedly decreased the number of primordial and primary follicles, while addition of sunflower oil reduced the number of primary follicles in 12-week-old rabbits. The number of antral follicles was higher following algae supplementation, but lower after addition of soybean oil in that age group. Proliferating index was decreased following supplementation with algae and soybean oil in juvenile rabbits, whereas it was increased after addition of algae and decreased following vegetable oils in mature ones. Dietary PUFAs did not impact apoptosis in the rabbit ovary of both age groups. The obtained results suggest that PUFA-enriched diet regulate either early folliculogenesis or antral follicle development in rabbits that might influence reproductive performance as a consequence. It appears that observed effects are attributed to sexual maturity.

这项研究旨在检查藻类（富含omega-3脂肪酸），葵花籽油（富含omega-6脂肪酸）和大豆油（富含omega-6脂肪酸）对青少年和性行为整个卵泡形成的影响成熟的兔子。断奶后，将兔子随机分为四个实验组，每组14只动物。对照动物接受未补充的颗粒，而在其他组中，颗粒包含1％的海藻，3％的葵花籽油或3％的大豆油。每组的动物在12周龄（每组n = 7）或18周龄（每组n = 7）处被屠宰。收获卵巢并固定以进行苏木精-伊红染色，PCNA的免疫组织化学定位和TUNEL分析。富含藻类的饮食显着减少了原始卵泡和初级卵泡的数量，而添加葵花籽油则减少了12周龄兔子的初级卵泡数量。在该年龄组，补充藻类后的窦房卵泡数量较高，而在添加大豆油后则较少。在幼兔中，添加藻类和大豆油后，增殖指数降低，而在添加藻类后，成熟兔中的增殖指数下降，而添加植物油后则下降。膳食PUFAs均不影响两个年龄组的兔卵巢细胞凋亡。获得的结果表明，富含PUFA的饮食可调节家兔的早期卵泡生成或胃窦卵泡发育，从而可能因此影响生殖性能。看来观察到的影响归因于性成熟。在该年龄组，补充藻类后的窦房卵泡数量较高，而在添加大豆油后则较少。在幼兔中，添加藻类和大豆油后，增殖指数降低，而在添加藻类后，成熟兔中的增殖指数下降，而添加植物油后则下降。膳食PUFAs均不影响两个年龄组的兔卵巢细胞凋亡。获得的结果表明，富含PUFA的饮食可调节家兔的早期卵泡生成或胃窦卵泡发育，从而可能因此影响生殖性能。看来观察到的效果归因于性成熟。在该年龄组，补充藻类后的窦房卵泡数量较高，而在添加大豆油后则较少。在幼兔中，添加藻类和大豆油后，增殖指数降低，而在添加藻类后，成熟兔中的增殖指数下降，而添加植物油后则下降。膳食PUFAs均不影响两个年龄组的兔卵巢细胞凋亡。获得的结果表明，富含PUFA的饮食可调节家兔的早期卵泡生成或胃窦卵泡发育，从而可能因此影响生殖性能。看来观察到的影响归因于性成熟。在幼兔中，添加藻类和大豆油后，增殖指数降低，而在添加藻类后，成熟兔中的增殖指数下降，而添加植物油后则下降。膳食PUFAs均不影响两个年龄组的兔卵巢细胞凋亡。获得的结果表明，富含PUFA的饮食可调节家兔的早期卵泡生成或胃窦卵泡发育，从而可能因此影响生殖性能。看来观察到的影响归因于性成熟。在幼兔中，添加藻类和大豆油后，增殖指数降低，而在添加藻类后，成熟兔中的增殖指数下降，而添加植物油后则下降。膳食PUFAs均不影响两个年龄组的兔卵巢细胞凋亡。获得的结果表明，富含PUFA的饮食可调节家兔的早期卵泡生成或胃窦卵泡发育，从而可能因此影响生殖性能。看来观察到的影响归因于性成熟。获得的结果表明，富含PUFA的饮食可调节家兔的早期卵泡生成或胃窦卵泡发育，从而可能因此影响生殖性能。看来观察到的影响归因于性成熟。获得的结果表明，富含PUFA的饮食可调节家兔的早期卵泡生成或胃窦卵泡发育，从而可能因此影响生殖性能。看来观察到的影响归因于性成熟。