Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results. Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation. Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs). The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models.

中文翻译：

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从大型家畜中产生诱导多能干细胞。


诱导多能干细胞（iPSC）在发育生物学研究和细胞治疗中具有巨大潜力。尽管在人类和小鼠模型中进行了广泛的研究和表征，但来自小鼠以外的动物的 iPSC 缺乏可重复的结果。在此，我们描述了通过慢病毒转导鼠或人转录因子 Oct4、Sox2、Klf4 和 c-Myc 从马和牛细胞中生成强大的 iPSC，以及使用类似的方案从人和鼠细胞中生成强大的 iPSC，即使使用不同的补充剂也是如此。对 iPSC 的形态、多能因子的基因和蛋白质表达、碱性磷酸酶检测以及自发和诱导分化进行了分析。尽管在家畜中胚胎干细胞尚未得到很好的表征，但通过与小鼠或人类细胞类似的方案，可以从这些物种中产生 iPS 细胞，从而能够将来自大型动物的多能细胞用于基本或应用目的。在此，我们还推断牛 iPS (biPSC) 与小鼠 iPSC (miPSC) 相似，而马 iPS (eiPSC) 与人类 (hiPSC) 相似。在不同动物物种中生成可重复的方案将为从家畜中产生体外自体多能细胞提供信息工具。这些细胞将通过转基因技术为动物育种创造新的机会，并将支持大型动物模型转化医学的新时代。