Medulloblastoma (MB) is one of the most common malignant cancers in children. MB is primarily classified into four subgroups based on molecular and clinical characteristics as (1) WNT (2) Sonic-hedgehog (SHH) (3) Group 3 (4) Group 4. Molecular characteristics used for MB classification are based on genomic and mRNAs profiles. MB subgroups share genomic and mRNA profiles and require multiple molecular markers for differentiation from each other. Long non-coding RNAs (lncRNAs) are more than 200 nucleotide long RNAs and primarily involve in gene regulation at epigenetic and post-transcriptional levels. LncRNAs have been recognized as diagnostic and prognostic markers in several cancers. However, the lncRNA expression profile of MB is unknown. We used the publicly available gene expression datasets for the profiling of lncRNA expression across MB subgroups. Functional analysis of differentially expressed lncRNAs was accomplished by Ingenuity pathway analysis (IPA). In the current study, we have identified and validated the lncRNA expression profile across pediatric MB subgroups and associated molecular pathways. We have also identified the prognostic significance of lncRNAs and unique lncRNAs associated with each MB subgroup. Identified lncRNAs can be used as single biomarkers for molecular identification of MB subgroups that warrant further investigation and functional validation.

中文翻译：

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儿科髓母细胞瘤的长期非编码RNA分析。

髓母细胞瘤（MB）是儿童中最常见的恶性肿瘤之一。根据分子和临床特征，MB主要分为四个亚组：（1）WNT（2）声波刺猬（SHH）（3）第3组（4）第4组。用于MB分类的分子特征基于基因组和mRNA。个人资料。MB亚组共享基因组和mRNA谱，并且需要多个分子标记来彼此区分。长非编码RNA（lncRNA）是200多个核苷酸长的RNA，主要参与表观遗传和转录后水平的基因调控。LncRNA被公认为几种癌症的诊断和预后标志物。然而，MB的lncRNA表达谱是未知的。我们使用可公开获得的基因表达数据集对MB亚组中的lncRNA表达进行分析。差异表达的lncRNA的功能分析通过独创性途径分析（IPA）完成。在当前的研究中，我们已经确定并验证了小儿MB亚组和相关分子途径的lncRNA表达谱。我们还确定了与每个MB亚组相关的lncRNA和独特的lncRNA的预后意义。鉴定出的lncRNA可用作MB亚组分子鉴定的单一生物标记，值得进一步研究和功能验证。我们已经确定并验证了小儿MB亚组和相关分子途径的lncRNA表达谱。我们还确定了与每个MB亚组相关的lncRNA和独特的lncRNA的预后意义。鉴定出的lncRNA可用作MB亚组分子鉴定的单一生物标记，值得进一步研究和功能验证。我们已经确定并验证了小儿MB亚组和相关分子途径的lncRNA表达谱。我们还确定了与每个MB亚组相关的lncRNA和独特的lncRNA的预后意义。鉴定出的lncRNA可用作MB亚组分子鉴定的单一生物标记，值得进一步研究和功能验证。