Colistin is considered as a last resort treatment for infections caused by multidrug-resistant Enterobacteriaceae. Plasmid-mediated mobile colistin resistance (mcr) genes contributed to the global spread of colistin resistance. This is the first report of plasmid-mediated colistin resistance mcr-8 gene from a clinical Klebsiella pneumoniae K9 isolate recovered from Lebanon. The isolate was characterized phenotypically and genotypically through both short and long read whole-genome sequencing, plasmid typing and conjugation assays. k9 belonged to sequence type 15 and harbored 31 antimicrobial resistance genes. The mcr-8.1 variant was carried on a novel ~ 300 kb multireplicon plasmid having IncFIA, IncR and IncHI1B. The plasmid was conjugative and carried a plethora of antimicrobial resistance determinants. The introduction of novel mcr variants in Lebanon poses an alarming health concern. Surveillance and screening for colistin resistant Enterobacteriaceae and mcr in livestock, animal farms, imported meat and poultry is highly recommended along with monitoring antibiotic use.

中文翻译：

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来自黎巴嫩的临床肺炎克雷伯菌的质粒介导的大肠菌素抗性mcr-8.1基因的首次报道。

Colistin被认为是由多重耐药肠杆菌科细菌引起的感染的最后手段。质粒介导的大肠粘菌素抗性（mcr）基因促成大肠粘菌素抗性的全球传播。这是从黎巴嫩回收的临床肺炎克雷伯菌K9分离株中质粒介导的大肠菌素抗性mcr-8基因的首次报道。通过短期和长期阅读的全基因组测序，质粒分型和缀合测定，对分离物进行了表型和基因型分析。k9属于序列类型15，包含31个抗菌素耐药基因。mcr-8.1变异体携带在具有IncFIA，IncR和IncHI1B的新型〜300 kb多复制子质粒上。该质粒是结合性的，并带有过多的抗药性决定簇。黎巴嫩引入了新颖的mcr变体，引起了令人担忧的健康问题。强烈建议在牲畜，动物农场，进口的肉类和家禽中进行对大肠菌素抗性肠杆菌科和mcr的监测和筛查，并监测抗生素的使用。